. "Pomoc\u00ED \u0159\u00EDzen\u00E9 metageneze (Quickchange, Stratagene) byly v cDNA pro extracelul\u00E1rn\u00ED \u010D\u00E1st ligandu TRAIL (aminokyseliny 95-281) c\u00EDlen\u011B mutov\u00E1ny dv\u011B (DR5 specifick\u00FD mutant), respektive \u010Dty\u0159i (DR4 specifick\u00FD mutant) aminokyseliny a po sekven\u010Dn\u00EDm ov\u011B\u0159en\u00ED vnesen\u00FDch mutac\u00ED byla exprese mutovan\u00FDch protein\u016F po p\u0159eklonov\u00E1n\u00ED do prokaryotick\u00E9ho expresn\u00EDho vektoru ove\u0159ena v E. coli (BL21)"@cs . "realiz\u00E1tor Apronex s.r.o., Nad Safinou II/365, 252 42 Jesenice u Prahy" . . "RIV/68378050:_____/08:00320648!RIV09-AV0-68378050" . . . "[BA3C55450148]" . "364733" . . "1"^^ . "E. coli expresn\u00ED plasmidy produkuj\u00EDc\u00ED TRAIL-R1/DR4 a TRAIL-R2/DR5 specifick\u00E9 mutanty lidsk\u00E9ho ligandu TRAIL" . . "TRAIL-R1/DR4; TRAIL-R2/DR5; expression vector"@en . . . "RIV/68378050:_____/08:00320648" . "E. coli expression plasmids producing TRAIL-R1/DR4- and TRAIL-R2/DR5-specific mutant sof the human ligand TRAIL"@en . "pEThTRAIL-EC-DR4m, -DR5m" . "1"^^ . . . "Using site-directed mutagenesis (Quickchange, Stratagene) were into cDNA encoding the extracellular part of the ligand TRAIL (amino acids 95-281) introduced two (DR5 specific) and four (DR4 specific) point mutations. Introduced mutations were verified by sequencing and the mutated TRAILs were after subcloning into an E. coli expression plasmid produced in E. coli(BL21) and purified."@en . . . . . "E. coli expression plasmids producing TRAIL-R1/DR4- and TRAIL-R2/DR5-specific mutant sof the human ligand TRAIL"@en . "E. coli expresn\u00ED plasmidy produkuj\u00EDc\u00ED TRAIL-R1/DR4 a TRAIL-R2/DR5 specifick\u00E9 mutanty lidsk\u00E9ho ligandu TRAIL" . . "P(1M0506), Z(AV0Z50520514)" . "E. coli expresn\u00ED plasmidy produkuj\u00EDc\u00ED TRAIL-R1/DR4 a TRAIL-R2/DR5 specifick\u00E9 mutanty lidsk\u00E9ho ligandu TRAIL"@cs . "Pomoc\u00ED \u0159\u00EDzen\u00E9 metageneze (Quickchange, Stratagene) byly v cDNA pro extracelul\u00E1rn\u00ED \u010D\u00E1st ligandu TRAIL (aminokyseliny 95-281) c\u00EDlen\u011B mutov\u00E1ny dv\u011B (DR5 specifick\u00FD mutant), respektive \u010Dty\u0159i (DR4 specifick\u00FD mutant) aminokyseliny a po sekven\u010Dn\u00EDm ov\u011B\u0159en\u00ED vnesen\u00FDch mutac\u00ED byla exprese mutovan\u00FDch protein\u016F po p\u0159eklonov\u00E1n\u00ED do prokaryotick\u00E9ho expresn\u00EDho vektoru ove\u0159ena v E. coli (BL21)" . "E. coli expresn\u00ED vektory produkuj\u00EDc\u00ED His-tagovan\u00E9 DR4 a DR5 specifick\u00E9 mutanty cytotoxick\u00E9ho ligandu TRAIL" . "V\u00FDsledek p\u0159ipraven ke komer\u010Dn\u00EDmu vyu\u017Eit\u00ED" . . "E. coli expresn\u00ED plasmidy produkuj\u00EDc\u00ED TRAIL-R1/DR4 a TRAIL-R2/DR5 specifick\u00E9 mutanty lidsk\u00E9ho ligandu TRAIL"@cs . . . . . "And\u011Bra, Ladislav" . .