. . . . "RIV/68378050:_____/08:00320585!RIV10-MSM-68378050" . "P(1M0506), Z(AV0Z50520514)" . "Human cell line Wnt1-9-II producing functional recombinant ligand Wnt1"@en . "Human cell line Wnt1-9-II producing functional recombinant ligand Wnt1"@en . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED ligand Wnt1"@cs . . . "V\u00FDsledek p\u0159ipraven ke komer\u010Dn\u00EDmu vyu\u017Eit\u00ED" . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED ligand Wnt1" . "376742" . . "1"^^ . "realiz\u00E1tor Apronex s.r.o., Nad Safinou II/365, 252 42 Jesenice u Prahy" . . "LLidsk\u00E1 bun\u011B\u010Dn\u00E1 linie 293 N3S se stabiln\u011B integrovan\u00FDm plazmidem, kter\u00FD exprimuje ligand Wnt1" . "Using standard molecular biological techniques we prepared a clone of human 293 NS3 cells producing human recombinant protein DKK1. The DKK proteins function as inhibitors of the Wnt signaling pathway, and, moreover these proteins can suppress growth of cancer cells in vitro and also in vivo. DKK1-4-II cells can be used as a valuable source of DKK1 ligand that might be used as potential anti-cancer agent."@en . . . . "Ko\u0159\u00EDnek, Vladim\u00EDr" . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED ligand Wnt1" . . . . . "1"^^ . . "Pomoc\u00ED molekul\u00E1rn\u011B biologick\u00FDch metod byl p\u0159ipraven expresn\u00ED plazmid k\u00F3duj\u00EDc\u00ED my\u0161\u00ED sekretovan\u00FD protein Wnt1. Tento plazmid byl transfekc\u00ED zaveden do lidsk\u00FDch bun\u011Bk 293 N3S. Jednotliv\u00E9 klony t\u011Bchto bun\u011Bk byly selektov\u00E1ny kultivac\u00ED v r\u016Fstov\u00E9m m\u00E9diu obsahuj\u00EDc\u00ED selek\u010Dn\u00ED antibiotikum (G418; expresn\u00ED plazmid k\u00F3duje gen pro rezistenci v\u016F\u010Di tomuto antibiotiku). Byl vyselektov\u00E1n bun\u011B\u010Dn\u00FD klon Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED ligand Wnt1. Proteiny rodiny Wnt byly pops\u00E1ny jako r\u016Fstov\u00E9 faktory nezbytn\u00E9 pro udr\u017Een\u00ED tzv. kmenovosti embryon\u00E1ln\u00EDch i somatick\u00FDch kmenov\u00FDch bun\u011Bk. Linie Wnt1-9-II je tedy vhodn\u00FDm zdrojem pro produkci v\u011Bt\u0161\u00EDho mno\u017Estv\u00ED tohoto proteinu. Ten je pak mo\u017En\u00E9 vyu\u017E\u00EDt v \u0159ad\u011B d\u016Fle\u017Eit\u00FDch aplikac\u00ED jako nap\u0159\u00EDklad p\u0159i izolac\u00EDch kmenov\u00FDch bun\u011Bk r\u016Fzn\u00E9ho p\u016Fvodu."@cs . . "[8AF5C5B4A45C]" . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED ligand Wnt1"@cs . "RIV/68378050:_____/08:00320585" . "human cell line 293 N3S; Wnt1; recombinant protein"@en . "Wnt1-9-II" . . "Pomoc\u00ED molekul\u00E1rn\u011B biologick\u00FDch metod byl p\u0159ipraven expresn\u00ED plazmid k\u00F3duj\u00EDc\u00ED my\u0161\u00ED sekretovan\u00FD protein Wnt1. Tento plazmid byl transfekc\u00ED zaveden do lidsk\u00FDch bun\u011Bk 293 N3S. Jednotliv\u00E9 klony t\u011Bchto bun\u011Bk byly selektov\u00E1ny kultivac\u00ED v r\u016Fstov\u00E9m m\u00E9diu obsahuj\u00EDc\u00ED selek\u010Dn\u00ED antibiotikum (G418; expresn\u00ED plazmid k\u00F3duje gen pro rezistenci v\u016F\u010Di tomuto antibiotiku). Byl vyselektov\u00E1n bun\u011B\u010Dn\u00FD klon Wnt1-9-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED ligand Wnt1. Proteiny rodiny Wnt byly pops\u00E1ny jako r\u016Fstov\u00E9 faktory nezbytn\u00E9 pro udr\u017Een\u00ED tzv. kmenovosti embryon\u00E1ln\u00EDch i somatick\u00FDch kmenov\u00FDch bun\u011Bk. Linie Wnt1-9-II je tedy vhodn\u00FDm zdrojem pro produkci v\u011Bt\u0161\u00EDho mno\u017Estv\u00ED tohoto proteinu. Ten je pak mo\u017En\u00E9 vyu\u017E\u00EDt v \u0159ad\u011B d\u016Fle\u017Eit\u00FDch aplikac\u00ED jako nap\u0159\u00EDklad p\u0159i izolac\u00EDch kmenov\u00FDch bun\u011Bk r\u016Fzn\u00E9ho p\u016Fvodu." . . . .