. "Human cell line DKK1-4-II producing functional recombinant protein DKK1"@en . "P(1M0506), Z(AV0Z50520514)" . "RIV/68378050:_____/08:00320583" . . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie 293 N3S se stabiln\u011B integrovan\u00FDm plazmidem, kter\u00FD exprimuje protein DKK1" . . . . . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED protein DKK1"@cs . "realiz\u00E1tor Apronex s.r.o., Nad Safinou II/365, 252 42 Jesenice u Prahy" . "Pomoc\u00ED molekul\u00E1rn\u011B biologick\u00FDch metod byl p\u0159ipraven expresn\u00ED plazmid k\u00F3duj\u00EDc\u00ED lidsk\u00FD sekretovan\u00FD protein DKK1. Tento plazmid byl transfekc\u00ED zaveden do lidsk\u00FDch bun\u011Bk 293 N3S. Jednotliv\u00E9 klony t\u011Bchto bun\u011Bk byly selektov\u00E1ny kultivac\u00ED v r\u016Fstov\u00E9m m\u00E9diu obsahuj\u00EDc\u00ED selek\u010Dn\u00ED antibiotikum (puromycin; expresn\u00ED plazmid k\u00F3duje gen pro rezistenci v\u016F\u010Di tomuto antibiotiku). Byl vyselektov\u00E1n bun\u011B\u010Dn\u00FD klon DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED ligand DKK1. Tento ligand je mo\u017En\u00E9 izolovat z kultiva\u010Dn\u00EDho m\u00E9dia za podm\u00EDnek, p\u0159i kter\u00FDch je zachov\u00E1na jeho biologick\u00E1 aktivita. Proteiny rodiny DKK1 funguj\u00ED jako inhibitory signalizace Wnt a jsou schopn\u00E9 potla\u010Dovat r\u016Fst n\u00E1dorov\u00FDch bun\u011Bk v kultur\u00E1ch (in vitro) i v \u017Eiv\u00FDch organismech. Linie DKK1-4-II je tedy vhodn\u00FDm zdrojem pro produkci v\u011Bt\u0161\u00EDho mno\u017Estv\u00ED tohoto proteinu, kter\u00FD je pot\u00E9 mo\u017Eno vyu\u017E\u00EDt p\u0159i studiu mechanism\u016F n\u00E1dorov\u00E9 transformace bun\u011Bk a potenci\u00E1ln\u011B i pro protin\u00E1dorov\u00E9 terapie." . . . . "Pomoc\u00ED molekul\u00E1rn\u011B biologick\u00FDch metod byl p\u0159ipraven expresn\u00ED plazmid k\u00F3duj\u00EDc\u00ED lidsk\u00FD sekretovan\u00FD protein DKK1. Tento plazmid byl transfekc\u00ED zaveden do lidsk\u00FDch bun\u011Bk 293 N3S. Jednotliv\u00E9 klony t\u011Bchto bun\u011Bk byly selektov\u00E1ny kultivac\u00ED v r\u016Fstov\u00E9m m\u00E9diu obsahuj\u00EDc\u00ED selek\u010Dn\u00ED antibiotikum (puromycin; expresn\u00ED plazmid k\u00F3duje gen pro rezistenci v\u016F\u010Di tomuto antibiotiku). Byl vyselektov\u00E1n bun\u011B\u010Dn\u00FD klon DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED ligand DKK1. Tento ligand je mo\u017En\u00E9 izolovat z kultiva\u010Dn\u00EDho m\u00E9dia za podm\u00EDnek, p\u0159i kter\u00FDch je zachov\u00E1na jeho biologick\u00E1 aktivita. Proteiny rodiny DKK1 funguj\u00ED jako inhibitory signalizace Wnt a jsou schopn\u00E9 potla\u010Dovat r\u016Fst n\u00E1dorov\u00FDch bun\u011Bk v kultur\u00E1ch (in vitro) i v \u017Eiv\u00FDch organismech. Linie DKK1-4-II je tedy vhodn\u00FDm zdrojem pro produkci v\u011Bt\u0161\u00EDho mno\u017Estv\u00ED tohoto proteinu, kter\u00FD je pot\u00E9 mo\u017Eno vyu\u017E\u00EDt p\u0159i studiu mechanism\u016F n\u00E1dorov\u00E9 transformace bun\u011Bk a potenci\u00E1ln\u011B i pro protin\u00E1dorov\u00E9 terapie."@cs . . "[AED826B24D2D]" . . "Ko\u0159\u00EDnek, Vladim\u00EDr" . . . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED protein DKK1" . "Human cell line DKK1-4-II producing functional recombinant protein DKK1"@en . . "human cell line 293 N3S; DKK1; recombinant protein"@en . . "Using standard molecular biological techniques we prepared a clone of human 293 NS3 cells producing human recombinant protein DKK1. The DKK proteins function as inhibitors of the Wnt signaling pathway, and, moreover these proteins can suppress growth of cancer cells in vitro and also in vivo. DKK1-4-II cells can be used as a valuable source of DKK1 ligand that might be used as potential anti-cancer agent."@en . . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED protein DKK1" . "V\u00FDsledek p\u0159ipraven ke komer\u010Dn\u00EDmu vyu\u017Eit\u00ED" . "1"^^ . "RIV/68378050:_____/08:00320583!RIV10-MSM-68378050" . "376741" . . "DKK1-4-II" . . "1"^^ . . "Lidsk\u00E1 bun\u011B\u010Dn\u00E1 linie DKK1-4-II produkuj\u00EDc\u00ED funk\u010Dn\u00ED rekombinantn\u00ED protein DKK1"@cs . . .