. "Posp\u00ED\u0161ek, M." . "88" . . "P(GA204/03/1487), P(GA301/07/0607), S, Z(AV0Z50520514), Z(MSM0021620813)" . "Journal of General Virology" . "Intern\u00ED ribosom\u00E1ln\u00ED vstupn\u00ED m\u00EDsto viru hepatitidy C zahajuje proteosynt\u00E9zu na autentick\u00E9m inicia\u010Dn\u00EDm kodonu v kvasink\u00E1ch"@cs . . "A novel yeast bicistronic reporter system, suitable for sensitive and accurate analysis of IRES activity, has been developed. By using this novel bicistronic system, it was shown that the HCV IRES is functional in yeast cells. Mutational analysis of the IRES loop IV and the adjacent region revealed that, in yeast, as in mammalian cells, translation initiates preferentially at the authentic (342)AUG codon and that disruption of the HCV IRES loop IV abrogates its function, whilst minor positional changes or substitutions of the initiation codon within loop IV are largely tolerated. These findings bring more general insights to translation initiation, but also open the door for utilization of yeast and its sophisticated genetics for searching for new antiviral drugs and HCV IRES trans-acting proteins." . "Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast"@en . "1"^^ . "Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "A novel yeast bicistronic reporter system, suitable for sensitive and accurate analysis of IRES activity, has been developed. By using this novel bicistronic system, it was shown that the HCV IRES is functional in yeast cells. Mutational analysis of the IRES loop IV and the adjacent region revealed that, in yeast, as in mammalian cells, translation initiates preferentially at the authentic (342)AUG codon and that disruption of the HCV IRES loop IV abrogates its function, whilst minor positional changes or substitutions of the initiation codon within loop IV are largely tolerated. These findings bring more general insights to translation initiation, but also open the door for utilization of yeast and its sophisticated genetics for searching for new antiviral drugs and HCV IRES trans-acting proteins."@en . . . "0022-1317" . "1992;2002" . . "11"^^ . "Vortelov\u00E1, L." . "[CF2AB40E536C]" . "423985" . "6"^^ . "IRES; hepatitida C; kvasinky"@en . . "7" . . "Vop\u00E1lensk\u00FD, V." . . . "Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast"@en . "RIV/68378050:_____/07:00094004!RIV08-AV0-68378050" . "Ma\u0161ek, T." . "Horv\u00E1th, Ond\u0159ej" . . . . . . . "RIV/68378050:_____/07:00094004" . . . . "Hepatitis C virus internal ribosome entry site initiates protein synthesis at the authentic initiation codon in yeast" . "Intern\u00ED ribosom\u00E1ln\u00ED vstupn\u00ED m\u00EDsto viru hepatitidy C zahajuje proteosynt\u00E9zu na autentick\u00E9m inicia\u010Dn\u00EDm kodonu v kvasink\u00E1ch"@cs . "Feketov\u00E1, Z." . "Byl vyvinut nov\u00FD bicistronick\u00FD reporterov\u00FD syst\u00E9m vhodn\u00FD pro citlivou a p\u0159esnou anal\u00FDzu aktivity IRES. S vyu\u017Eit\u00EDm tohoto syst\u00E9mu bylo uk\u00E1z\u00E1no, \u017Ee IRES viru HCV funguje v bu\u0148k\u00E1ch kvasinek. Muta\u010Dn\u00ED anal\u00FDza uk\u00E1zala, \u017Ee v kvasink\u00E1ch, stejn\u011B jako v sav\u010D\u00EDch bu\u0148k\u00E1ch, je translace iniciov\u00E1na preferen\u010Dn\u011B na (342)AUG kodonu a \u017Ee naru\u0161en\u00ED HCV IRS smy\u010Dky IV ru\u0161\u00ED jeho funkci, zat\u00EDmco men\u0161\u00ED muta\u010Dn\u00ED zm\u011Bny ve smy\u010Dce IV jsou vcelku tolerov\u00E1ny. yto n\u00E1lezy p\u0159in\u00E1\u0161ej\u00ED obecn\u011B lep\u0161\u00ED pochopen\u00ED iniciace translace, ale otev\u00EDraj\u00ED tak\u00E9 mo\u017Enost k vyu\u017Eit\u00ED kvasinek a jejich sofistikovan\u00E9 genetiky k hled\u00E1n\u00ED nov\u00FDch virostatik a HCV IRES trans-acting protein\u016F."@cs .