. "Tepl\u00E1, O." . "P\u011Bknicov\u00E1, Jana" . "P(1M06011), P(LC545), Z(AV0Z50520514), Z(AV0Z50520701)" . "421409" . "0015-0282" . "88" . . . . . . "Sulimenko, Tetyana" . "Fertility and Sterility" . . "beta-tubulin; sperm head; Pathological spermiogram"@en . "Zral\u00E9 spermie maj\u00ED jasnou strukturu, kter\u00E1 je ve vztahu k cytoskelet\u00E1ln\u00EDm protein\u016Fm, kter\u00E9 maj\u00ED funk\u010Dn\u00ED \u00FAlohu v procesu fertilizace a motilit\u011B spermi\u00ED. V p\u0159edchoz\u00EDch publikac\u00EDch jsme prok\u00E1zali neo\u010Dek\u00E1van\u011B jasnou detekci \u03B2-tubulin v hlavi\u010Dce kan\u010D\u00EDch a lidsk\u00FDch spermi\u00ED pomoc\u00ED protil\u00E1tky TU-12. V t\u00E9to pr\u00E1ci byl jasn\u011B detekov\u00E1n epitop tubulinov\u00E9 molekuly a rozd\u00EDly v jeho detekci u lidsk\u00FDch spermi\u00ED s norm\u00E1ln\u00EDm a patologick\u00FDm spermiogramem. Spermie byly rozd\u011Bleny do p\u011Bti kategori\u00ED normozoospermia, oligozoospermia, asthenozoospermia, teratozoospermia a astheno-teratozoospermia. TU-12 epitop byl lokalizov\u00E1n v \u03B2-tubulinov\u00E9 oblasti \u03B2426-435. Immunobloting prok\u00E1zal kvantitativn\u00ED rozd\u00EDly v mno\u017Estv\u00ED tubulinu mezi mu\u017Ei s norm\u00E1ln\u00EDm a patologick\u00FDcm spermiogramem. Podobn\u011B byly prok\u00E1zany i rozd\u00EDly v expozici TU-12 epitopu v hlavi\u010Dce spermi\u00ED u norm\u00E1ln\u00EDch a patologick\u00FDch spermi\u00ED. V\u00FDsledky prok\u00E1zaly mo\u017Enost pou\u017Eit\u00ED tubulinov\u00E9ho epitopu jako biomarkeru pro stanoven\u00ED patologick\u00E9ho stavu spermi\u00ED."@cs . "Koubek, Pavel" . "Mature spermatozoon exhibits extraordinary structural compartmentalization due to the presence of cytoskeletal proteins and functions in fertilization and motility. We have shown previously that anti-\u03B2-tubulin antibody TU-12 provided unexpectedly strong reaction in human and boar sperm head. Now we wanted to determine location of the corresponding epitope on the tubulin molecule and to find any differences in its exposition in human sperm with normal and pathological spermiograms. Sperm were divided into 5 categories: normozoo-, oligozoo-, asthenozoo-, teratozoo- and astheno-teratozoospermia, and well-characterized monoclonal antibodies were applied to monitor tubulin epitope distributions. TU-12 epitope was located in \u03B2-tubulin region \u03B2426-435. Immunoblotting revealed differences in the amount of tubulin among men with normal and pathological spermiograms. Striking differences were observed especially in sperm heads. Tubulin epitopes could thus be useful biomarkers of sperm pathology."@en . "Expression of beta-tubulin epitope in human sperm with pathological spermiogram" . "Exprese beta-tubulinov\u00E9ho epitopu v lidsk\u00FDch spermi\u00EDch s patologick\u00FDm spermiogramem"@cs . "RIV/68378050:_____/07:00093368" . . . . "RIV/68378050:_____/07:00093368!RIV08-AV0-68378050" . . . . "Exprese beta-tubulinov\u00E9ho epitopu v lidsk\u00FDch spermi\u00EDch s patologick\u00FDm spermiogramem"@cs . . "Mature spermatozoon exhibits extraordinary structural compartmentalization due to the presence of cytoskeletal proteins and functions in fertilization and motility. We have shown previously that anti-\u03B2-tubulin antibody TU-12 provided unexpectedly strong reaction in human and boar sperm head. Now we wanted to determine location of the corresponding epitope on the tubulin molecule and to find any differences in its exposition in human sperm with normal and pathological spermiograms. Sperm were divided into 5 categories: normozoo-, oligozoo-, asthenozoo-, teratozoo- and astheno-teratozoospermia, and well-characterized monoclonal antibodies were applied to monitor tubulin epitope distributions. TU-12 epitope was located in \u03B2-tubulin region \u03B2426-435. Immunoblotting revealed differences in the amount of tubulin among men with normal and pathological spermiograms. Striking differences were observed especially in sperm heads. Tubulin epitopes could thus be useful biomarkers of sperm pathology." . "Pexidrov\u00E1, Marie" . "1120;1128" . . "Expression of beta-tubulin epitope in human sperm with pathological spermiogram" . . . . . "7"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Expression of beta-tubulin epitope in human sperm with pathological spermiogram"@en . . "Kub\u00E1tov\u00E1, Alena" . "9"^^ . "6"^^ . "Dr\u00E1ber, Pavel" . "Expression of beta-tubulin epitope in human sperm with pathological spermiogram"@en . "[520F18A17082]" . . . . "2" .