. "[AACF2D3E2B6D]" . "Tich\u00E1, M." . "IE - Irsko" . . . "Monoklon\u00E1ln\u00ED protil\u00E1tky (MAbs) proti DQH proteinu byly p\u0159ipraveny a pou\u017Eity pro ur\u010Den\u00ED p\u016Fvodu DQH v reproduk\u010Dn\u00EDch org\u00E1nech kance, jeho lokalizace na spermi\u00EDch a pro sledov\u00E1n\u00ED vazebn\u00FDch schopnost\u00ED v prase\u010D\u00EDm oviduktu a se ZP vaj\u00ED\u010Dka. mRNA transkript DQH byl nalezen v semenn\u00FDch vac\u00EDch (SV) ne v testes, epididymu a prostat\u011B. Translatovan\u00E9 produkty byly pomoc\u00ED MAbs imunodetekov\u00E1ny v extraktu a tekutin\u011B SV, na tk\u00E1\u0148ov\u00FDch \u0159ezech SV a na membr\u00E1n\u011B na akrosomu ejakulovan\u00FDch spermi\u00ED. V\u00FDsledky potvrzuj\u00ED schopnost vazby DQH proteinu na povrch spermie p\u0159i ejakulaci a \u00FA\u010Dast na tvorb\u011B reservo\u00E1ru spermi\u00ED v prase\u010D\u00EDm oviduktu. Mabs redukuj\u00ED tak\u00E9 vazbu spermi\u00ED k vaj\u00ED\u010Dku a potvrzuj\u00ED tak roli DQH proteinu v prim\u00E1rn\u00ED vazb\u011B spermie se ZP vaj\u00ED\u010Dka."@cs . . . . . "11"^^ . . . . "1-2" . "P\u016Fvod, lokalizace a vazebn\u00E9 vlastnosti kan\u010D\u00EDho DQH povrchov\u00E9ho proteinu spermie testovan\u00E9 specifick\u00FDmi monoklon\u00E1ln\u00EDmi protil\u00E1tkami"@cs . . . "Journal of Reproductive Immunology" . "Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies"@en . "RIV/68378050:_____/07:00093159!RIV08-AV0-68378050" . . . . "Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies" . "P(1M06011), P(GA303/06/0895), P(GP303/04/P070), Z(AV0Z50520514), Z(AV0Z50520701)" . "P\u016Fvod, lokalizace a vazebn\u00E9 vlastnosti kan\u010D\u00EDho DQH povrchov\u00E9ho proteinu spermie testovan\u00E9 specifick\u00FDmi monoklon\u00E1ln\u00EDmi protil\u00E1tkami"@cs . "Boar DQH sperm surface protein; monoclonal antibody; binding abilities in the porcine oviduct"@en . "Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies" . "4"^^ . . "RIV/68378050:_____/07:00093159" . "P\u011Bknicov\u00E1, Jana" . "440054" . "103;113" . "74" . . . "0165-0378" . . . "Ma\u0148\u00E1skov\u00E1, Pavla" . . "Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the ZP of the oocyte. The mRNA transcript of DQH was found in seminal vesicles, not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in seminal vesicle extract and fluid, on seminal vesicle tissue sections and on the membrane-associated acrosome part of ejaculated spermatozoa. These results confirmed the ability of DQH protein to bind the sperm surface at ejaculation and to participate in the formation of the sperm reservoir in the porcine oviduct. Moreover, Mabs reduced binding of sperm to oocytes and proved the role of DQH protein in the sperm-zona pellucida primary binding."@en . "Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the ZP of the oocyte. The mRNA transcript of DQH was found in seminal vesicles, not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in seminal vesicle extract and fluid, on seminal vesicle tissue sections and on the membrane-associated acrosome part of ejaculated spermatozoa. These results confirmed the ability of DQH protein to bind the sperm surface at ejaculation and to participate in the formation of the sperm reservoir in the porcine oviduct. Moreover, Mabs reduced binding of sperm to oocytes and proved the role of DQH protein in the sperm-zona pellucida primary binding." . "5"^^ . "Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies"@en . . . "Elzeinov\u00E1, Fatima" . "Jon\u00E1kov\u00E1, V\u011Bra" .