. "1"^^ . "Maurer, D." . "TCR; dendritic cells; Src kinases"@en . "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation" . . "2262;2271" . "We show that individual cognate pMHC complexes displayed by dendritic cells (DCs), as compared with nonprofessional APCs, are far better in productively triggering Ag-specific TCRs independently of conventional costimulation. As we further show, this is accomplished by the unique ability of DCs to robustly activate the Src family kinases (SFKs) Lck and Fyn in T cells even in the absence of cognate peptide. Instead, this form of SFK activation depends on interactions of DC-displayed MHC with TCRs of appropriate restriction, suggesting a central role of self-pMHC recognition. DC-mediated SFK activation leads to %22TCR licensing,%22 a process that dramatically increases sensitivity and magnitude of the TCR response to cognate pMHC. Thus, TCR licensing, besides costimulation, is a main mechanism of DCs to present Ag effectively."@en . "Komplexy pMHC na povrchu dendritick\u00FDch bun\u011Bk (DC) jsou ve srovn\u00E1n\u00ED s jin\u00FDmi typy neprofesion\u00E1ln\u00EDch APC mnohem \u00FA\u010Dinn\u011Bj\u0161\u00ED ve stimulaci antigenn\u011B-specifick\u00FDch T lymfocyt\u016F, nez\u00E1visle na konven\u010Dn\u00ED kostimulaci. To je zp\u016Fsobeno unik\u00E1tn\u00ED schopnost\u00ED DC robustn\u011B aktivovat v T lymfocytech kinasy skupiny Src (SFK) Lck a Fyn, a to dokonce v nep\u0159\u00EDtomnosti specifick\u00E9ho pMHC. Tato forma aktivace SFK z\u00E1vis\u00ED na interakc\u00EDch MHC na povrchu DC s TCR, co\u017E poukazuje na \u00FAst\u0159edn\u00ED \u00FAlohu rozpozn\u00E1n\u00ED self-MHC. Aktivace SFK mediovan\u00E1 DC vede k \u201ETCR licensing\u201C, co\u017E je proces, kter\u00FD dramaticky zvy\u0161uje citlivost a velikost odpov\u011Bdi TCR na pMHC. TCR licensing je tedy krom\u011B kostimulace hlavn\u00EDm mechanismem zodpov\u011Bdn\u00FDm za efektivn\u00ED prezentaci antigenu pomoc\u00ED DC."@cs . "[A8528C5CCA27]" . "Dendritick\u00E9 bu\u0148ky senzitizuj\u00ED TCR prost\u0159ednictv\u00EDm aktivace kin\u00E1z skupiny Src a vlastn\u00EDch MHC protein\u016F"@cs . "Ho\u0159ej\u0161\u00ED, V\u00E1clav" . "Journal of Immunology" . . "178" . "We show that individual cognate pMHC complexes displayed by dendritic cells (DCs), as compared with nonprofessional APCs, are far better in productively triggering Ag-specific TCRs independently of conventional costimulation. As we further show, this is accomplished by the unique ability of DCs to robustly activate the Src family kinases (SFKs) Lck and Fyn in T cells even in the absence of cognate peptide. Instead, this form of SFK activation depends on interactions of DC-displayed MHC with TCRs of appropriate restriction, suggesting a central role of self-pMHC recognition. DC-mediated SFK activation leads to %22TCR licensing,%22 a process that dramatically increases sensitivity and magnitude of the TCR response to cognate pMHC. Thus, TCR licensing, besides costimulation, is a main mechanism of DCs to present Ag effectively." . "6"^^ . "Stingl, G." . . . "Z(AV0Z50520514)" . "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation"@en . "Fischer, G. F." . "10"^^ . "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation"@en . . "Dendritick\u00E9 bu\u0148ky senzitizuj\u00ED TCR prost\u0159ednictv\u00EDm aktivace kin\u00E1z skupiny Src a vlastn\u00EDch MHC protein\u016F"@cs . "4" . "Meraner, P." . "416189" . "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation" . . . "Wolpl, A." . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . . "0022-1767" . "RIV/68378050:_____/07:00085144" . "RIV/68378050:_____/07:00085144!RIV08-AV0-68378050" . . .