"Na\u010Fov\u00E1, Zuzana" . "525687" . "apoptosis; iron deprivation; mitochondrial apoptotic pathway"@en . "2" . . "Truksa, Jaroslav" . . "Iron deprivation induces apoptosis via mitochondrial changes related to Bax translocation" . "10" . . . "Deprivace \u017Eeleza indukuje apopt\u00F3zu skrze mitochondri\u00E1ln\u00ED zm\u011Bny souvisej\u00EDc\u00ED s Bax translokac\u00ED"@cs . . . "Iron deprivation induces apoptosis via mitochondrial changes related to Bax translocation" . . . . . "Deprivace \u017Eeleza indukuje apopt\u00F3zu skrze mitochondri\u00E1ln\u00ED zm\u011Bny souvisej\u00EDc\u00ED s Bax translokac\u00ED"@cs . . . . "RIV/68378050:_____/05:00027661" . "5"^^ . . . "Ehrlichov\u00E1, Marie" . "Kov\u00E1\u0159, Jan" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "In order to elucidate the mechanisms involved in apoptosis induction by iron deprivation, we compared cells sensitive (38C13) and resistant (EL4) to apoptosis induced by iron deprivation. Iron deprivation was achieved by incubation in a defined iron-free medium. We detected the activation of caspase-3 and caspase-9 only in sensitive cells under iron deprivation. Iron deprivation led to the release of cytochrome c from mitochondria into the cytosol only in sensitive cells. The mitochondrial membrane potential (MMP) was dissipated within 24 h in sensitive cells due to iron deprivation. The antiapoptotic Bcl-2 protein was found to be associated with mitochondria in both sensitive and resistant cells and the association did not change under iron deprivation. However, under iron deprivation we detected translocation of the proapoptotic Bax protein from the cytosol to mitochondria only in sensitive cells."@en . . "5"^^ . . "Abychom objasnili mechanismus uplatn\u011Bn\u00FD v indukci apopt\u00F3zy deprivac\u00ED \u017Eeleza, srovn\u00E1vali jsme bu\u0148ky sensitivn\u00ED (38C13) a resistentn\u00ED (EL4) k bun\u011B\u010Dn\u00E9 smrti indukovan\u00E9 deprivac\u00ED \u017Eeleza. Deprivaci \u017Eeleza jsme navodili kultivaci bun\u011Bk v bez\u017Eelez\u00E9m m\u00E9diu. V podm\u00EDnk\u00E1ch deprivace \u017Eeleza jsme u sensitivn\u00EDch bun\u011Bk detekovali aktivaci kasp\u00E1zy 3 a kasp\u00E1zy 9. Deprivace \u017Eeleza u sensitivn\u00EDch bun\u011Bk d\u00E1le vedla k uvoln\u011Bn\u00ED cytochromu c z mitochondri\u00ED do cytosolu. V d\u016Fsledku deprivace \u017Eeleza do\u0161lo u sensitivn\u00EDch bun\u011Bk k zhroucen\u00ED mitochondri\u00E1ln\u00EDho membr\u00E1nov\u00E9ho potenci\u00E1lu (MMP) b\u011Bhem 24 h. Antiapoptotick\u00FD protein Bax byl nalezen na mitochondri\u00EDch jak u sensitivn\u00EDch tak i resistentn\u00EDch bun\u011Bk a tato asociace s mitochondriemi se vlivem deprivace \u017Eeleza nezm\u011Bnila. Av\u0161ak za podm\u00EDnek deprivace \u017Eeleza jsme detekovali translokaci apoptotick\u00E9ho Bax proteinu z cytosolu na mitochondrie u sensitivn\u00EDch bun\u011Bk k indukci apopt\u00F3zy."@cs . . "Apoptosis" . . "13"^^ . "P(GA301/01/0041), Z(AV0Z5052915)" . "1360-8185" . . "In order to elucidate the mechanisms involved in apoptosis induction by iron deprivation, we compared cells sensitive (38C13) and resistant (EL4) to apoptosis induced by iron deprivation. Iron deprivation was achieved by incubation in a defined iron-free medium. We detected the activation of caspase-3 and caspase-9 only in sensitive cells under iron deprivation. Iron deprivation led to the release of cytochrome c from mitochondria into the cytosol only in sensitive cells. The mitochondrial membrane potential (MMP) was dissipated within 24 h in sensitive cells due to iron deprivation. The antiapoptotic Bcl-2 protein was found to be associated with mitochondria in both sensitive and resistant cells and the association did not change under iron deprivation. However, under iron deprivation we detected translocation of the proapoptotic Bax protein from the cytosol to mitochondria only in sensitive cells." . "381;393" . "RIV/68378050:_____/05:00027661!RIV06-AV0-68378050" . "Iron deprivation induces apoptosis via mitochondrial changes related to Bax translocation"@en . "[381BF3C45E13]" . "Koc, Michal" . "Iron deprivation induces apoptosis via mitochondrial changes related to Bax translocation"@en .