"RIV/68378050:_____/05:00026205" . "8"^^ . "\u0160tokrov\u00E1, Jitka" . "Hol\u00E1\u0148, Vladim\u00EDr" . "536702" . "10"^^ . "RIV/68378050:_____/05:00026205!RIV06-AV0-68378050" . "Pseudokapsidy polyoma viru EGFP: anal\u00FDza modelov\u00FDch partikul\u00ED pro vn\u00E1\u0161en\u00ED protein\u016F a peptid\u016F do sav\u010D\u00EDch bun\u011Bk"@cs . "Liebl, D." . "A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terimal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion." . . . . . "Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells"@en . "29" . "Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells" . . . . "3"^^ . . "Sp\u00ED\u0161ek, R." . "Pseudokapsidy polyoma viru EGFP: anal\u00FDza modelov\u00FDch partikul\u00ED pro vn\u00E1\u0161en\u00ED protein\u016F a peptid\u016F do sav\u010D\u00EDch bun\u011Bk"@cs . "0014-5793" . "Forstov\u00E1, J." . "Bou\u0159a, E." . "Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells" . . "Fri\u010D, Jan" . "579" . . "Byl zkonstruov\u00E1n vektor my\u0161\u00EDch polyomavirov\u00FDch kapsid-like \u010D\u00E1stic pro p\u0159enos cizorod\u00FDch peptid\u016F a protein\u016F do bun\u011Bk. Byly p\u0159ipraveny modelov\u00E9 pseudokapsidy nesouc\u00ED EGFP fuzovan\u00FD s C-termin\u00E1ln\u00ED \u010D\u00E1st\u00ED minoritn\u00EDho proteinu VP3 (EGFP-VPLs) a byla analyzov\u00E1na jejich schopnost internalizace a zpracov\u00E1n\u00ED my\u0161\u00EDmi bu\u0148kami jako\u017E i schopnost aktivace my\u0161\u00EDch a lidsk\u00FDch dendritick\u00FDch bun\u011Bk in vitro. EGFP-VPLs \u00FA\u010Dinn\u011B pronikly do my\u0161\u00EDch epiteli\u00E1ln\u00EDch bun\u011Bk, fibroblast\u016F a lidsk\u00FDch i my\u0161\u00EDch dendritick\u00FDch bun\u011Bk a byly upravov\u00E1ny jak lysozomy tak proteazomy. P\u0159ekvapiv\u011B v\u0161ak neindukovaly stimulaci molekul a matura\u010Dn\u00EDch marker\u016F in vitro, ale indukovaly sekreci interleukinu 12."@cs . "Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells"@en . . "FEBS Letters" . . . "[E17BEC8AE34D]" . . "6549;6558" . . "Marek, M." . "P(GA204/03/0593), P(NC6957), Z(AV0Z50520514)" . . "mouse polyomavirus; empty artificial virus-like particle; dendritic cell activation"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . . "A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terimal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion."@en .