"5"^^ . . "0024-4066" . . "Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes"@en . "Vl\u010Dek, \u010Cestm\u00EDr" . "Pozi\u010Dn\u00ED klonov\u00E1n\u00ED genu Hybrid sterility 1: genetick\u00E9 mapov\u00E1n\u00ED a evaluace dvou kandid\u00E1tn\u00EDch gen\u016F"@cs . . "Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes" . . "Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes" . "The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs."@en . "Himmelbauer, H." . "Mihola, Ond\u0159ej" . . . . "Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes"@en . . "RIV/68378050:_____/05:00000229" . . "Gen Hybrid sterility 1 (Hst1) ovliv\u0148uje fertilitu hybridn\u00EDch samc\u016F mezi n\u011Bkter\u00FDmi laboratorn\u00EDmi kmeny (nap\u0159. C57BL/10) a my\u0161\u00ED (pod)druhu Mus musculus musculus. B\u011Bhem pozi\u010Dn\u00EDho klonov\u00E1n\u00ED Hst1 genu jsme vygenerovali kontig bakteri\u00E1ln\u00EDch artifici\u00E1ln\u00EDch chromosom\u016F (BAC) a vzorkovou sekvenci kandid\u00E1tn\u00ED oblasti ze 129S1/SvImJ kmene. Nov\u00E9 genetick\u00E9 markery n\u00E1m umo\u017Enily z\u00FA\u017Eit Hst1 oblast z 580 na 360 kb. Produkty dvou gen\u016F z t\u00E9to oblasti, TATA-vazebn\u00FD protein (Tbp) a proteasomov\u00E1 podjednotka beta 1 (Psmb1), akumuluj\u00ED b\u011Bhem spermatogeneze. Tyto proteiny maj\u00ED konzervovan\u00E9 C-terminaln\u00ED sekvence a druhov\u011B-specifick\u00E9 N-konce. Vyhodnotili jsme kandidaturu t\u011Bchto gen\u016F na gen Hst1 alelick\u00FDm sekvenov\u00E1n\u00EDm a semikvantitativn\u00EDm RT PCR v re\u00E1ln\u00E9m \u010Dase pro jejich testikul\u00E1rn\u00ED mRNA."@cs . "Trachtulec, Zden\u011Bk" . . "The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs." . "Pa\u010Des, V\u00E1clav" . . "Forejt, Ji\u0159\u00ED" . "Pozi\u010Dn\u00ED klonov\u00E1n\u00ED genu Hybrid sterility 1: genetick\u00E9 mapov\u00E1n\u00ED a evaluace dvou kandid\u00E1tn\u00EDch gen\u016F"@cs . . . "house mouse; Mus musculus; speciation"@en . . "-" . . "RIV/68378050:_____/05:00000229!RIV/2005/AV0/A23005/N" . "P(GA204/01/0997), P(GV204/98/K015), P(LN00A079), Z(AV0Z50520514), Z(AV0Z5052915)" . . "[9138793B8974]" . "536898" . . . "84" . "637;641" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "6"^^ . . . "5"^^ . . . "Biological Journal of the Linnean Society" . .