. "3"^^ . "RIV/68378050:_____/04:00109714" . "monoclonal antibody; human spermatozoa; sperm protein"@en . . . "P\u011Bknicov\u00E1, Jana" . "Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding" . . "The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein" . . "Celistvost akrozomu spermie je nezbytn\u00E1 pro norm\u00E1ln\u00ED interakci spermie s vaj\u00ED\u010Dkem. K ov\u011B\u0159en\u00ED stavu akrozomu se je\u0161t\u011B st\u00E1le \u010Dasto pou\u017E\u00EDvaj\u00ED protil\u00E1tky proti protein\u016Fm akrozomu. Studovali jsme akrozom\u00E1ln\u00ED proteiny pomoc\u00ED protil\u00E1tek Hs-8 a Hs-14 p\u0159ipraven\u00FDch v na\u0161\u00ED laborato\u0159i. Proteiny uvnit\u0159 akrozomu rozpoznan\u00E9 Hs-8 a Hs-14 jsou p\u0159i kapacitaci a dokonce i po indukci akrozomov\u00E9 reakce stabiln\u00ED. Byla zkoum\u00E1na \u00FAloha protein\u016F pHs-8 a pHs-14 ve vazb\u011B spermie na vaj\u00ED\u010Dko pomoc\u00ED vazebn\u00E9ho testu kan\u010D\u00EDch spermi\u00ED a prase\u010D\u00EDch oocyt\u016F in vitro. Protein zji\u0161t\u011Bny Hs-14 se vazby gamet ne\u00FA\u010Dastn\u00ED, zat\u00EDmco protein detekovan\u00FD Hs-8 je proteinem sekund\u00E1rn\u00ED vazby"@cs . . "1046-7408" . "The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein"@en . . "P(GA204/02/1373), P(NJ7463)" . "\u010Capkov\u00E1, Jana" . . . "Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding" . "51" . "Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding"@en . . . "2"^^ . "Geussov\u00E1, Gizela" . . "480;481" . "Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding"@en . . . "6" . "Celistvost akrozomu sav\u010D\u00EDch spermi\u00ED hodnocen\u00E1 monoklon\u00E1ln\u00EDmi protil\u00E1tkami proti vnit\u0159n\u00EDm protein\u016Fm akrozomu a \u00FAloha t\u011Bchto protein\u016F ve vazb\u011B spermie a vaj\u00ED\u010Dka"@cs . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "RIV/68378050:_____/04:00109714!RIV/2005/GA0/A23005/N" . "Celistvost akrozomu sav\u010D\u00EDch spermi\u00ED hodnocen\u00E1 monoklon\u00E1ln\u00EDmi protil\u00E1tkami proti vnit\u0159n\u00EDm protein\u016Fm akrozomu a \u00FAloha t\u011Bchto protein\u016F ve vazb\u011B spermie a vaj\u00ED\u010Dka"@cs . "[1DC8E1E9E6A3]" . "3"^^ . . "American Journal of Reproductive Immunology" . "553431" . . . .