. "RIV/68378050:_____/04:00109003" . "1136;1145" . . . "567310" . "I-mediated signaling events by Lyn kinase C-terminal tyrosine phosphorylation" . . . "0014-2980" . . . . . . "RIV/68378050:_____/04:00109003!RIV/2005/AV0/A23005/N" . "10"^^ . . . "Dr\u00E1ber, Petr" . "Tolarov\u00E1, Helena" . . "I-mediated signaling events by Lyn kinase C-terminal tyrosine phosphorylation"@en . "[BCBB5835A657]" . . "Using a phospho-Lyn-specific antiserum, we show an increased phosphorylation of the Lyn C-terminal regulatory tyrosine and decreased Lyn kinase activity during Fc epsilon RI-mediated mast cell activation. Mutant Lyn, defective in the C-terminal tyrosine, constitutively phosphorylated several substrates in resting cells, but did not cause Fc epsilon RI internalization or spontaneous degranulation. Fc epsilon RI-induced signaling in the presence of constitutively active Lyn exhibited enhanced phosphorylation of the receptor subunits, Syk, LAT, Gab2, phospholipase C (PLC)gamma 1 and PLC gamma 2, and production of phosphatidylinositol 3,4,5-trisphosphate. Although enzymatic activities of PLC gamma 1 and PLC gamma 2 were also up-regulated, amounts of inositol 1,4,5-trisphosphate, mobilization of intracellular calcium and degranulation were suppressed. Additionally, constitutively active Lyn was strikingly less efficient than wild-type Lyn in restoring the receptor-mediated calcium respon..." . "34" . . "I-mediated signaling events by Lyn kinase C-terminal tyrosine phosphorylation" . "Pozitivn\u00ED a negativn\u00ED regulace signaliza\u010Dn\u00EDch d\u011Bj\u016F zprost\u0159edkovan\u00FDch Fcepsilon receptorem I pomoc\u00ED fosforylace C-koncov\u00E9ho tyrozinu kin\u00E1zy Lyn"@cs . "mast cell; Fc receptor; signal transduction"@en . "I-mediated signaling events by Lyn kinase C-terminal tyrosine phosphorylation"@en . . "European Journal of Immunology" . "Using a phospho-Lyn-specific antiserum, we show an increased phosphorylation of the Lyn C-terminal regulatory tyrosine and decreased Lyn kinase activity during Fc epsilon RI-mediated mast cell activation. Mutant Lyn, defective in the C-terminal tyrosine, constitutively phosphorylated several substrates in resting cells, but did not cause Fc epsilon RI internalization or spontaneous degranulation. Fc epsilon RI-induced signaling in the presence of constitutively active Lyn exhibited enhanced phosphorylation of the receptor subunits, Syk, LAT, Gab2, phospholipase C (PLC)gamma 1 and PLC gamma 2, and production of phosphatidylinositol 3,4,5-trisphosphate. Although enzymatic activities of PLC gamma 1 and PLC gamma 2 were also up-regulated, amounts of inositol 1,4,5-trisphosphate, mobilization of intracellular calcium and degranulation were suppressed. Additionally, constitutively active Lyn was strikingly less efficient than wild-type Lyn in restoring the receptor-mediated calcium respon..."@en . "4"^^ . . . "4" . . "4"^^ . . . "Pozitivn\u00ED a negativn\u00ED regulace signaliza\u010Dn\u00EDch d\u011Bj\u016F zprost\u0159edkovan\u00FDch Fcepsilon receptorem I pomoc\u00ED fosforylace C-koncov\u00E9ho tyrozinu kin\u00E1zy Lyn"@cs . "Tolar, Pavel" . . "Dr\u00E1berov\u00E1, Lubica" . . . "Pomoc\u00ED antis\u00E9ra specifick\u00E9ho pro fosfo-Lyn ukazujeme zv\u00FD\u0161enou fosforylaci C-koncov\u00E9ho regula\u010Dn\u00EDho tyrozinu Lyn a sn\u00ED\u017Eenou aktivitu kin\u00E1zy Lyn b\u011Bhem aktivace \u017E\u00EDrn\u00FDch bun\u011Bk zprost\u0159edkovan\u00E9 Fc epsilon RI. Mutovan\u00E1 Lyn s chyb\u011Bj\u00EDc\u00EDm C-koncov\u00FDm tyrozinem konstitutivn\u011B fosforylovala \u0159adu substr\u00E1t\u016F v klidov\u00FDch bu\u0148k\u00E1ch, ale nevedla k internalizaci Fe epsilon RI \u010Di ke spont\u00E1nn\u00ED degranulaci. Signalizace vyvolan\u00E1 Fc epsilon RI za p\u0159\u00EDtomnosti konstitutivn\u011B aktivn\u00ED Lyn vykazovala zv\u00FD\u0161enou fosforylaci receptorov\u00FDch podjednotek Syk, LAT, Gab2, fosfolip\u00E1zy C(PLC)gama 1 a PLC gama 2 a produkci fosfatidylinositol 3,4,5-trifosf\u00E1tu. I kdy\u017E byly enzymatick\u00E9 aktivity PLC gama 1 a PLC gama 2 tak\u00E9 zv\u00FD\u0161eny, mno\u017Estv\u00ED inositol 1,4,5-trifosf\u00E1tu, mobilizace intracelul\u00E1rn\u00EDho v\u00E1pn\u00EDku a degranulace byly sn\u00ED\u017Eeny. Nav\u00EDc byla konstitutivn\u011B aktivn\u00ED Lyn v\u00FDrazn\u011B m\u00E9n\u011B \u00FA\u010Dinn\u00E1 p\u0159i obnov\u011B odpov\u011Bd\u00ED v\u00E1pn\u00EDku zprost\u0159edkovan\u00FDch receptorem v \u017E\u00EDrn\u00FDch bu\u0148k\u00E1ch kostn\u00ED d\u0159en\u011B odvozen\u00FDch z Lyn(-/-) my\u0161\u00ED ne\u017E Lyn divok\u00E9ho typu. Tyto poznat..."@cs . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "P(GA204/00/0204), P(GA204/03/0594), P(GA301/03/0596), P(GA310/00/0205), P(IAA5052005), P(IAA5052310), P(IAA7052006), P(LN00A026), Z(AV0Z5052915)" . . .