"\u0160\u00EDmov\u00E1, \u0160\u00E1rka" . "13"^^ . "Wilson, B. S." . . . "Luskov\u00E1, Petra" . . . . . "12"^^ . . "Dr\u00E1berov\u00E1, Lubica" . "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL" . . . . . "8"^^ . "Dr\u00E1ber, Petr" . . "Ho\u0159ej\u0161\u00ED, V\u00E1clav" . "Negativn\u00ED regulace signalizace a funkce \u017E\u00EDrn\u00FDch bun\u011Bk zprost\u0159edkovan\u00E1 adaptorem LAB/NTAL"@cs . . "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL"@en . . . "Lebdu\u0161ka, Pavel" . . "To clarify the role of NTAL in mast cell degranulation, we compared FcepsilonRI-mediated signaling events in BMMCs from NTAL-deficient and wild-type mice. Although NTAL is structurally similar to LAT, antigen-mediated degranulation responses were unexpectedly increased in NTAL-deficient mast cells. The earliest event affected was enhanced tyrosine phosphorylation of LAT in antigen-activated cells. This was accompanied by enhanced tyrosine phosphorylation and enzymatic activity of phospholipase C gamma1 and phospholipase C gamma2, resulting in elevated levels of inositol 1,4,5-trisphosphate and free intracellular Ca2+. NTAL-deficient BMMCs also exhibited an enhanced activity of phosphatidylinositol 3-OH kinase and Src homology 2 domain-containing protein tyrosine phosphatase-2. Although both LAT and NTAL are considered to be localized in membrane rafts, immunogold electron microscopy on isolated membrane sheets demonstrated their independent clustering. The combined data show that NT..." . . . . "575666" . . "200" . . "RIV/68378050:_____/04:00108999" . "To clarify the role of NTAL in mast cell degranulation, we compared FcepsilonRI-mediated signaling events in BMMCs from NTAL-deficient and wild-type mice. Although NTAL is structurally similar to LAT, antigen-mediated degranulation responses were unexpectedly increased in NTAL-deficient mast cells. The earliest event affected was enhanced tyrosine phosphorylation of LAT in antigen-activated cells. This was accompanied by enhanced tyrosine phosphorylation and enzymatic activity of phospholipase C gamma1 and phospholipase C gamma2, resulting in elevated levels of inositol 1,4,5-trisphosphate and free intracellular Ca2+. NTAL-deficient BMMCs also exhibited an enhanced activity of phosphatidylinositol 3-OH kinase and Src homology 2 domain-containing protein tyrosine phosphatase-2. Although both LAT and NTAL are considered to be localized in membrane rafts, immunogold electron microscopy on isolated membrane sheets demonstrated their independent clustering. The combined data show that NT..."@en . "K objasn\u011Bn\u00ED \u00FAlohy NTAL v degranulaci \u017E\u00EDrn\u00FDch bun\u011Bk jsme srovnali signaliza\u010Dn\u00ED kroky zprost\u0159edkovan\u00E9 FcepsilonRI v bu\u0148k\u00E1ch BMMC z my\u0161\u00ED s chyb\u011Bj\u00EDc\u00EDm NTAL a my\u0161\u00ED divok\u00E9ho typu. I kdy\u017E je NTAL struktur\u00E1ln\u011B podobn\u00FD LAT, degranula\u010Dn\u00ED odpov\u011Bdi zprost\u0159edkovan\u00E9 antigenem byly u \u017E\u00EDrn\u00FDch bun\u011Bk s chyb\u011Bj\u00EDc\u00EDm NTAL ne\u010Dekan\u011B zv\u00FD\u0161en\u00E9. Nej\u010Dasn\u011Bji zasa\u017Een\u00FDm krokem byla zv\u00FD\u0161en\u00E1 fosforylace tyrozinu LAT v bu\u0148k\u00E1ch aktivovan\u00FDch antigenem. Sou\u010Dasn\u011B s t\u00EDm byla zv\u00FD\u0161ena fosforylace tyrozinu a enzymatick\u00E1 aktivita fosfolip\u00E1zy C gama1 a fosfolip\u00E1zy C gama2, co\u017E vedlo ke zv\u00FD\u0161en\u00ED hladin inozitol-1,4,5-trifosf\u00E1tu a voln\u00E9ho intracelul\u00E1rn\u00EDho Ca2+. BMMC s chyb\u011Bj\u00EDc\u00EDm NTAL tak\u00E9 vykazovaly zv\u00FD\u0161enou aktivitu fosfatidyinozitol 3-OH kin\u00E1zy protein tyrozin fosfat\u00E1zy-2 obsahuj\u00EDc\u00ED dom\u00E9nu Src homologie 2. I kdy\u017E se p\u0159edpokl\u00E1d\u00E1, \u017Ee LAT i NTAL se nach\u00E1zej\u00ED v membr\u00E1nov\u00FDch ostr\u016Fvc\u00EDch, elektronov\u00E1 mikroskopie vyu\u017E\u00EDvaj\u00EDc\u00ED imunitn\u00ED reakce se zlatem na izolovan\u00FDch vrstv\u00E1ch membr\u00E1ny prok\u00E1zala jejich nez\u00E1visl\u00E9 shluky. Kombinovan\u00E1 data u..."@cs . "Heneberg, Petr" . "8" . "Mallisen, B." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Journal of Experimental Medicine" . "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL" . "Boubel\u00EDk, Michael" . "RIV/68378050:_____/04:00108999!RIV/2005/AV0/A23005/N" . "1001;1013" . "P(GA204/03/0594), P(GA301/03/0596), P(IAA5052310), P(LN00A026), Z(AV0Z5052915)" . "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL"@en . . . "mast cell; LAB/NTAL; cell signaling"@en . . "Negativn\u00ED regulace signalizace a funkce \u017E\u00EDrn\u00FDch bun\u011Bk zprost\u0159edkovan\u00E1 adaptorem LAB/NTAL"@cs . "Bugajev, V." . "Mallisen, M." . . . "0022-1007" . . "[6BD72DBD0D01]" .