. "1-2" . "Heat shock protein 90 (Hsp90) is a molecular chaperone abundant in eukaryotic cells. However, its exact role is not completely understood yet. Employing an iron-binding assay and mass spectrometric analysis, we have identified human Hsp90 as an iron-binding protein in membrane protein preparations of human HeLa cells. Western blot analysis and confocal microscopy confirmed that a portion of cellular Hsp90 is associated with the plasma membrane, but it does not seem to be expressed on the cell surface. The iron-binding assay with purified human Hsp90 confirmed iron binding by Hsp90. Thus we suggest that Hsp90 is an iron-binding protein ssociated with the plasma membrane"@en . "6"^^ . . . "P(GA301/01/0041), P(IAA5052702)" . "Heat shock protein 90 (Hsp90) is a molecular chaperone abundant in eukaryotic cells. However, its exact role is not completely understood yet. Employing an iron-binding assay and mass spectrometric analysis, we have identified human Hsp90 as an iron-binding protein in membrane protein preparations of human HeLa cells. Western blot analysis and confocal microscopy confirmed that a portion of cellular Hsp90 is associated with the plasma membrane, but it does not seem to be expressed on the cell surface. The iron-binding assay with purified human Hsp90 confirmed iron binding by Hsp90. Thus we suggest that Hsp90 is an iron-binding protein ssociated with the plasma membrane" . "Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells" . "41;46" . . . "RIV/68378050:_____/04:00108974!RIV/2005/AV0/A23005/N" . "Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells" . . "1015-8987" . "Protein tepeln\u00E9ho \u0161oku 90 jako protein v\u00E1zaj\u00EDc\u00ED \u017Eelezo spojen\u00FD s plasmatickou membr\u00E1nou bun\u011Bk HeLa"@cs . "14" . "Scheiber-Mojdehkar, B." . "Nov\u00E1k, J." . . . "Protein tepeln\u00E9ho \u0161oku 90 (Hsp90) je molekul\u00E1rn\u00ED chaperon hojn\u011B se vyskytuj\u00EDc\u00ED v eukaryotick\u00FDch bu\u0148k\u00E1ch. Jeho p\u0159esn\u00E1 \u00FAloha v\u0161ak je\u0161t\u011B nebyla zcela vyjasn\u011Bna. Pomoc\u00ED testu na vazbu \u017Eeleza a anal\u00FDzy hmotnostn\u00ED spektrometri\u00ED jsme v prepar\u00E1tech membr\u00E1nov\u00FDch protein\u016F z lidsk\u00FDch bun\u011Bk HeLa identifikovali lidsk\u00FD Hsp90 jako protein v\u00E1zaj\u00EDc\u00ED \u017Eelezo. Anal\u00FDza Westernova p\u0159enosu a konfok\u00E1ln\u00ED mikroskopie potvrdila, \u017Ee \u010D\u00E1st bun\u011B\u010Dn\u00E9ho Hsp90 je spojena s plasmatickou membr\u00E1nou, ale nen\u00ED z\u0159ejm\u011B exprimov\u00E1n na bun\u011B\u010Dn\u00E9m povrchu. Test na vazbu \u017Eeleza s purifikovan\u00FDm lidsk\u00FDm Hsp90 potvrdil, \u017Ee Hsp90 v\u00E1\u017Ee \u017Eelezo. Vyvozujeme z toho, \u017Ee Hsp90 je protein v\u00E1zaj\u00EDc\u00ED \u017Eelezo spojen\u00FD s plasmatickou membr\u00E1nou"@cs . . "Truksa, Jaroslav" . "[79CC642EBB31]" . . "Cellular Physiology and Biochemistry" . . "Kriegerbeckov\u00E1, Karin" . "RIV/68378050:_____/04:00108974" . "Ehrlichov\u00E1, Marie" . . . "Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells"@en . "6"^^ . . "Goldenberg, H." . "Koc, Michal" . "582870" . "Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells"@en . . "Kov\u00E1\u0159, Jan" . . . . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . "Protein tepeln\u00E9ho \u0161oku 90 jako protein v\u00E1zaj\u00EDc\u00ED \u017Eelezo spojen\u00FD s plasmatickou membr\u00E1nou bun\u011Bk HeLa"@cs . "9"^^ . . . "heat shock protein 90; iron-binding protein; plasma membrane"@en . . "\u0160t\u00FDbrov\u00E1, Hana" .