. . . . "NL - Nizozemsko" . "487;498" . . "P(GA304/03/0090), Z(AV0Z5052915)" . "565287" . . "Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk"@en . "Transgenic Research" . . "Miku\u0161, Tom\u00E1\u0161" . . "0962-8819" . "Trefil, P." . . "Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk" . . "[A6458A2107CB]" . "P\u0159\u00EDprava a fenotypov\u00E1 anal\u00FDza transgenn\u00ED linie kr\u00E1l\u00EDk\u016F sekretuj\u00EDc\u00EDch aktivn\u00ED rekombinantn\u00ED lidsk\u00FD erytropoietin v ml\u00E9ce"@cs . . "4"^^ . . . . "Production of recombinant human erythropoietin (rhEPO) for therapeutic purposes relies on its expression in selected clones of transfected mammalian cells. Alternatively, this glycoprotein can be produced by targeted secretion into the body fluid of transgenic mammals. Here we report on the generation of a transgenic rabbits producing rhEPO in the lactating mammary gland. Transgenic individuals are viable, fertile and transmit the rhEPO gene to the offspring. Northern blot data indicated that the expression of the transgene in the mammary gland is controlled by WAP regulatory sequences during the period of lactation. While the hybridization with total RNA revealed the expression only in the lactating mammary gland, the highly sensitive combinatory approach using RT-PCR/hybridization technique detected a minor ectopic expression. The level of rhEPO secretion in the founder female, measured in the period of lactation, varied in the range of 60-178 and 60-162 mUI/ml in the milk and blo..."@en . "5" . "13" . "Popl\u0161tein, M." . "erythropoietin, mammary gland, transgenic rabbit"@en . "RIV/68378050:_____/04:00105390!RIV/2005/AV0/A23005/N" . "8"^^ . "12"^^ . "P\u0159\u00EDprava a fenotypov\u00E1 anal\u00FDza transgenn\u00ED linie kr\u00E1l\u00EDk\u016F sekretuj\u00EDc\u00EDch aktivn\u00ED rekombinantn\u00ED lidsk\u00FD erytropoietin v ml\u00E9ce"@cs . "Jen\u00EDkov\u00E1, Gabriela" . "Sedl\u00E1kov\u00E1, J." . "Lidick\u00FD, J." . . . . "Mal\u00FD, Petr" . . "Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk"@en . "Produkce rekombinantn\u00EDho lidsk\u00E9ho erytropoietinu (rhEPO) pro l\u00E9\u010Debn\u00E9 \u00FA\u010Dely je zalo\u017Eena ne jaho expresi ve vybran\u00FDch klonech transfekovan\u00FDch sav\u010D\u00EDch bun\u011Bk. Tento glykoprotein m\u016F\u017Ee b\u00FDt tak\u00E9 produkov\u00E1n c\u00EDlenou sekrec\u00ED do t\u011Blesn\u00FDch tekutin transgenn\u00EDch zv\u00ED\u0159at. Zde popisujeme p\u0159\u00EDpravu transgenn\u00EDch kr\u00E1l\u00EDk\u016F produkuj\u00EDc\u00EDch rhEPO do laktuj\u00EDc\u00ED ml\u00E9\u010Dn\u00E9 \u017El\u00E1zy. Transgenn\u00ED jedinci jsou \u017Eivotaschopn\u00ED, plodn\u00ED a p\u0159ed\u00E1vaj\u00ED gen rhEPO potomstvu. \u00DAdaje z Northernova p\u0159enosu uk\u00E1zaly, \u017Ee exprese transgenu v ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze je b\u011Bhem laktace \u0159\u00EDzena regula\u010Dn\u00EDmi sekvencemi WAP. Zat\u00EDmco hybridizace s celkovou RNA uk\u00E1zala expresi pouze v laktuj\u00EDc\u00ED ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze, vysoce citliv\u00FD kombinovan\u00FD p\u0159\u00EDstup vyu\u017E\u00EDvaj\u00EDc\u00ED techniku RT-PCR/hybridizace odhalil slabou ektopickou expresi. \u00DArove\u0148 sekrece rhEPO v zakladatelsk\u00E9 transgenn\u00ED samici m\u011B\u0159en\u00E1 v dob\u011B laktace se pohybovala v rozmez\u00ED 60-178 a 60-162 mUI/ml v ml\u00E9ku resp. krevn\u00ED plazm\u011B. Biologick\u00E1 aktivita rhEPO z ml\u00E9ka byla potvrzena standardn\u00EDm testem inkorporace 3H-thymidinu. Popi..."@cs . "Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk" . . . "RIV/68378050:_____/04:00105390" . "Production of recombinant human erythropoietin (rhEPO) for therapeutic purposes relies on its expression in selected clones of transfected mammalian cells. Alternatively, this glycoprotein can be produced by targeted secretion into the body fluid of transgenic mammals. Here we report on the generation of a transgenic rabbits producing rhEPO in the lactating mammary gland. Transgenic individuals are viable, fertile and transmit the rhEPO gene to the offspring. Northern blot data indicated that the expression of the transgene in the mammary gland is controlled by WAP regulatory sequences during the period of lactation. While the hybridization with total RNA revealed the expression only in the lactating mammary gland, the highly sensitive combinatory approach using RT-PCR/hybridization technique detected a minor ectopic expression. The level of rhEPO secretion in the founder female, measured in the period of lactation, varied in the range of 60-178 and 60-162 mUI/ml in the milk and blo..." . "Landa, Vladim\u00EDr" .