. "Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction"@en . . . "Stamenova, M." . . . "Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction" . "16"^^ . . "Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction" . "sperm-egg interaction; antibodies"@en . "RIV/68378050:_____/04:00105370" . "RIV/68378050:_____/04:00105370!RIV/2005/GA0/A23005/N" . "Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction"@en . "Studium specifick\u00FDch a funk\u010Dn\u011B aktivn\u00EDch antigen\u016F spermi\u00ED by mohlo napomoci objasnit mechanismy interakce gamet a naj\u00EDt nov\u00E9 postupu progn\u00F3zy a regulace plodnosti. Vyu\u017Eili jsme polyklon\u00E1ln\u00ED kr\u00E1li\u010D\u00ED protikan\u010D\u00ED protil\u00E1tku (RABSA) k dvojit\u00E9 imunodif\u00FAzi, elektrofor\u00E9ze, imunofluorescen\u010Dn\u00EDmu testu a testu ELISA. Protil\u00E1tka RABSA barvila akrozomy. RABSA je specificky lokalizov\u00E1n ve spermatick\u00FDch struktur\u00E1ch. U Westernova p\u0159enosu rozpozn\u00E1vala RABSA specificky antigen s molekulovou hmotnost\u00ED 60 kDa (Ag60). Biologick\u00E9 a IIF experimenty prokazuj\u00ED, \u017Ee Ag60 se \u00FA\u010Dastn\u00ED funk\u010Dn\u00EDch krok\u016F nezbytn\u00FDch pro vazbu spermie na vaj\u00ED\u010Dko a/nebo jejich f\u00FAzi, ale ne pro vazbu spermie na zonu pellucidu"@cs . "1-2" . . "5"^^ . "Identifikace a izolace kan\u010D\u00EDch antigen\u016F specifick\u00FDch pro spermie s potenci\u00E1ln\u00ED \u00FAlohou v interakci spermie s vaj\u00ED\u010Dkem"@cs . . . "Identifikace a izolace kan\u010D\u00EDch antigen\u016F specifick\u00FDch pro spermie s potenci\u00E1ln\u00ED \u00FAlohou v interakci spermie s vaj\u00ED\u010Dkem"@cs . "Journal of Reproductive Immunology" . "Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding" . "1"^^ . . . "Baracova, V." . . "P(GA204/02/1373), P(NJ7463), Z(AV0Z5052915)" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding"@en . "64" . "91;106" . . "[0BD1DDFD6626]" . "0165-0378" . . "Mollova, M." . . "567134" . "Ivanova, M." . "P\u011Bknicov\u00E1, Jana" .