"Jon\u00E1k, Ji\u0159\u00ED" . "2004-06-24+02:00"^^ . . . "Faktory EF-Tu jsou hojn\u011B se vyskytuj\u00EDc\u00ED t\u0159\u00EDdom\u00E9nov\u00E9 GTP\u00E1zy pod\u00EDlej\u00EDc\u00ED se na translaci mRNA ve v\u0161ech zn\u00E1m\u00FDch mikroorganismech. Jejich dom\u00E9na 1 m\u00E1 GTP\u00E1zovou aktivitu, v\u00E1\u017Ee GDP a GTP a naz\u00FDv\u00E1 se dom\u00E9na G. Vysok\u00E1 homologie r\u016Fzn\u00FDch EF-Tu je p\u0159edur\u010Duje k objasn\u011Bn\u00ED strukturn\u00EDch vlastnost\u00ED adaptace na r\u016Fzn\u00E9 \u017Eivotn\u00ED podm\u00EDnky. EF-Tu z m\u00EDrn\u011B termostabiln\u00ED G+ bakterie Bacillus stearothermophilus (Bst), rostouc\u00ED p\u0159i cca 60\u00B0C, byla vybr\u00E1na k ur\u010Den\u00ED pod\u00EDlu jednotliv\u00FDch dom\u00E9n na jej\u00ED termostabilit\u011B, abychom zjistili, co zp\u016Fsobuje jeho vy\u0161\u0161\u00ED termostabilitu ve srovn\u00E1n\u00ED s EF-Tu z mezofiln\u00ED Escherichia coli (Ec). Za t\u00EDmto \u00FA\u010Delem byly p\u0159ipraveny chimerick\u00E9 formy EF-Tu slo\u017Een\u00E9 z mezofiln\u00EDch a termofiln\u00EDch dom\u00E9n a izolovan\u00FDch dom\u00E9n G a byly charakterizov\u00E1ny jejich funk\u010Dn\u00ED parametry a termostabilita. Bylo zji\u0161t\u011Bno, \u017Ee kone\u010Dn\u00E1 termostabilita obou EF-Tu je v\u00FDsledkem kooperativn\u00ED interakce mezi dom\u00E9nou G a dom\u00E9nami 2+3. Dom\u00E9na G nastavila %22z\u00E1kladn\u00ED%22 hladinu termostability, kter\u00E1 byla asi o 20\u00B0C vy\u0161\u0161\u00ED u dom\u00E9ny..."@cs . . "Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu" . "P(GA303/02/0689), Z(AV0Z5052915)" . . "Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu"@en . . "thermostability; EF-Tu; chimeric protein"@en . . . . "590261" . . "3"^^ . "Malo\u0148, Petr" . . . "EF-Tus are abundant three-domain GTPases involved in mRNA translation in all known microorganisms. Their domain 1 has a GTPase activity, binds GDP and GTP and is called the G-domain. The high homology of various EF-Tus predetermines them for the elucidation of structural features of adaptation to different living conditions. The EF-Tu from moderately thermostable G+ bacterium Bacillus stearothermophilus (Bst), growing around 60C, was chosen to determine the contribution of individual domains to its thermostability to learn what makes it more thermostable than EF-Tu from mesophilic Escherichia coli (Ec). For this purpose chimeric forms of EF-Tu composed of mesophilic and thermophilic domains and isolated G-domains were prepared and their functional parameters and thermostability were characterized. The final thermostability of either EF-Tu was found to be the result of a cooperative interaction between the G-domain and domains 2+3. The G-domain set up the \u00F4basic\u00F6 level of the thermos..." . "\u0160anderov\u00E1, Hana" . . "???"@cs . . . "H\u016Flkov\u00E1, Marta" . "RIV/68378050:_____/04:00105330!RIV/2005/AV0/A23005/N" . "4"^^ . . . "RIV/68378050:_____/04:00105330" . . "EF-Tus are abundant three-domain GTPases involved in mRNA translation in all known microorganisms. Their domain 1 has a GTPase activity, binds GDP and GTP and is called the G-domain. The high homology of various EF-Tus predetermines them for the elucidation of structural features of adaptation to different living conditions. The EF-Tu from moderately thermostable G+ bacterium Bacillus stearothermophilus (Bst), growing around 60C, was chosen to determine the contribution of individual domains to its thermostability to learn what makes it more thermostable than EF-Tu from mesophilic Escherichia coli (Ec). For this purpose chimeric forms of EF-Tu composed of mesophilic and thermophilic domains and isolated G-domains were prepared and their functional parameters and thermostability were characterized. The final thermostability of either EF-Tu was found to be the result of a cooperative interaction between the G-domain and domains 2+3. The G-domain set up the \u00F4basic\u00F6 level of the thermos..."@en . . "FEBS Forum For Young Scientists, Abstract book" . "FEBS" . . "Var\u0161ava" . "Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu" . "Var\u0161ava" . "[38772F748510]" . "???"@cs . "Thermostability of multidomain proteins: chimeric mesophilic/thermophilic elongation factors EF-Tu"@en . "7"^^ . "7" . .