"Zavadil, J." . . "Integrace p\u0159enosu sign\u00E1lu TGF-beta/Smad a Jagged1/Notch p\u0159i p\u0159echodu epiteli\u00E1ln\u00EDch bun\u011Bk v mezenchym\u00E1ln\u00ED"@cs . . "Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition"@en . "568451" . "Epithelial-to-mesenchymal transitions (EMTs) underlie cell plasticity required in embryonic development and frequently observed in advanced carcinogenesis. Transforming growth factor-beta (TGF-beta) induces EMT phenotypes in epithelial cells in vitro and has been associated with EMT in vivo. Here we report that expression of the hairy/enhancer-of-split-related transcriptional repressor Hey1, and the Notch-ligand Jagged1 (Jag1), was induced by TGF-beta at the onset of EMT in epithelial cells from mammary gland, kidney tubules, and epidermis. The HEY1 expression profile was biphasic, consisting of immediate-early Smad3-dependent, Jagged1/Notch-independent activation, followed by delayed, indirect Jagged1/Notch-dependent activation. TGF-beta-induced EMT was blocked by RNA silencing of HEY1 or JAG1, and by chemical inactivation of Notch. The EMT phenotype, biphasic activation of Hey1, and delayed expression of Jag1 were induced by TGF-beta in wild-type, but not in Smad3-deficient, prima..." . "1"^^ . "\u010Cerm\u00E1k, Luk\u00E1\u0161" . "RIV/68378050:_____/04:00105321" . . "4"^^ . "1155;1165" . "Soto-Nieves, N." . "Z(AV0Z5052915)" . "TGFb, Notch, EMT"@en . . "EMBO Journal" . "P\u0159echody bun\u011Bk z epiteli\u00E1ln\u00EDch na mezenchym\u00E1ln\u00ED (EMT) jsou podm\u00EDn\u011Bny plasticitou bun\u011Bk nezbytnou pro v\u00FDvoj embrya a jsou \u010Dasto pozorovan\u00E9 u pokro\u010Dil\u00E9 karcinogeneze. Transforma\u010Dn\u00ED r\u016Fstov\u00FD faktor beta (TGF-beta) vyvol\u00E1v\u00E1 fenotypy EMT v epiteli\u00E1ln\u00EDch bu\u0148k\u00E1ch in vitro a byl spojov\u00E1n s EMT in vivo. Zde ukazujeme, \u017Ee na po\u010D\u00E1tku EMT v epiteli\u00E1ln\u00EDch bu\u0148k\u00E1ch ml\u00E9\u010Dn\u00E9 \u017El\u00E1zy, ledvinov\u00FDch kan\u00E1lc\u00EDch a epidermis byla TGF-beta vyvol\u00E1na exprese transkrip\u010Dn\u00EDho represoru Hey1 (hairy/enhancer-of-split-related) a Notch-ligand Jagged1 (Jag1). Profil exprese HEY1 byl dvouf\u00E1zov\u00FD, sest\u00E1vaj\u00EDc\u00ED z velmi \u010Dasn\u00E9 aktivace z\u00E1visl\u00E9 na Smad3 a nez\u00E1visl\u00E9 na Jagged1/Notch, n\u00E1sledovan\u00E9 opo\u017Ed\u011Bnou, nep\u0159\u00EDmou aktivac\u00ED z\u00E1vislou na Jagged1/Notch. EMT vyvolan\u00FD TGF-beta byl blokov\u00E1n uml\u010Den\u00EDm HEY1 \u010Di JAG1 RNA a chemickou inaktivac\u00ED Notch. Fenotyp EMT, dvouf\u00E1zov\u00E1 aktivace Hey1 a opo\u017Ed\u011Bn\u00E1 exprese Jag1 byly vyvol\u00E1ny TGF-beta v epiteli\u00E1ln\u00EDch bu\u0148k\u00E1ch divok\u00E9ho typu, ale ne v prim\u00E1rn\u00EDch bu\u0148k\u00E1ch my\u0161\u00EDch ledvinov\u00FDch kan\u00E1lk\u016F s chyb\u011Bj\u00EDc\u00EDm S..."@cs . . "Bottinger, E. P." . . . "Epithelial-to-mesenchymal transitions (EMTs) underlie cell plasticity required in embryonic development and frequently observed in advanced carcinogenesis. Transforming growth factor-beta (TGF-beta) induces EMT phenotypes in epithelial cells in vitro and has been associated with EMT in vivo. Here we report that expression of the hairy/enhancer-of-split-related transcriptional repressor Hey1, and the Notch-ligand Jagged1 (Jag1), was induced by TGF-beta at the onset of EMT in epithelial cells from mammary gland, kidney tubules, and epidermis. The HEY1 expression profile was biphasic, consisting of immediate-early Smad3-dependent, Jagged1/Notch-independent activation, followed by delayed, indirect Jagged1/Notch-dependent activation. TGF-beta-induced EMT was blocked by RNA silencing of HEY1 or JAG1, and by chemical inactivation of Notch. The EMT phenotype, biphasic activation of Hey1, and delayed expression of Jag1 were induced by TGF-beta in wild-type, but not in Smad3-deficient, prima..."@en . "23" . "0261-4189" . "[14C0AF5B88EC]" . . "-" . "RIV/68378050:_____/04:00105321!RIV/2005/AV0/A23005/N" . "Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Integrace p\u0159enosu sign\u00E1lu TGF-beta/Smad a Jagged1/Notch p\u0159i p\u0159echodu epiteli\u00E1ln\u00EDch bun\u011Bk v mezenchym\u00E1ln\u00ED"@cs . . . . "Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition" . "11"^^ . . . "Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition" . .