. "308" . "2"^^ . "Isolation, characterization and binding properties of boar epididymal fluid proteins"@en . "RIV/68378050:_____/04:00105277!RIV/2005/AV0/A23005/N" . "Biology of Reproduction" . "2004-08-01+02:00"^^ . . . "569072" . "Isolation, characterization and binding properties of boar epididymal fluid proteins" . "Izolace, charakterizace a vazebn\u00E9 vlastnosti protein\u016F kan\u010D\u00ED epididym\u00E1ln\u00ED tekutiny"@cs . "[68C38973790D]" . . . . "RIV/68378050:_____/04:00105277" . . "Tich\u00E1, M." . "Society for the Study of Reproduction" . "P(GA303/02/0433), P(GP303/04/P070), P(NJ7463), Z(AV0Z5052915)" . . "The ability of some epididymal proteins to associate with spermatozoa suggests their role in sperm maturation and/or in consequential phases of the fertilization process. By size exclusion chromatography, RP HPLC and SDS-PAGE we separated proteins from boar epididymal fluid. By the zymography method, we detected serine- and metallo-proteinases. Epididymal fluid proteins were tested with biotinylated glycoproteins of zona pellucida (ZP), hyaluronic acid, and heparin on nitrocellulose membrane after Western blot. The affinity of some epididymal proteins with heparin and hyaluronic acid suggests their role in sperm capacitation in the oviduct. Protein bands interacted with ZP glycoproteins on the blot. This phenomenon might indicate a possible role of epididymal fluid proteins bound to the sperm surface in epididymal maturation and in the sperm-egg binding"@en . . "Isolation, characterization and binding properties of boar epididymal fluid proteins"@en . "Ma\u0148\u00E1skov\u00E1, Pavla" . "Izolace, charakterizace a vazebn\u00E9 vlastnosti protein\u016F kan\u010D\u00ED epididym\u00E1ln\u00ED tekutiny"@cs . "Isolation, characterization and binding properties of boar epididymal fluid proteins" . . . . "Vancouver" . . "Jon\u00E1kov\u00E1, V\u011Bra" . . . "Schopnost n\u011Bkter\u00FDch epididym\u00E1ln\u00EDch protein\u016F spojovat se se spermiemi nazna\u010Duje jejich \u00FAlohu p\u0159i zr\u00E1n\u00ED spermi\u00ED a/nebo n\u00E1sledn\u00FDch f\u00E1z\u00EDch oplod\u0148ovac\u00EDho procesu. Pomoc\u00ED chromatografie s vylu\u010Dov\u00E1n\u00EDm podle velikosti, RP HPLC a SDS-PAGE jsme rozd\u011Blili proteiny kan\u010D\u00ED epididym\u00E1ln\u00ED tekutiny. Zymografickou metodou jsme odhalili serinov\u00E9 protein\u00E1zy a metaloprotein\u00E1zy. Proteiny epididym\u00E1ln\u00ED tekutiny byly na nitrocelul\u00F3zov\u00E9 membr\u00E1n\u011B po Westernov\u011B p\u0159enosu testov\u00E1ny biotinylovan\u00FDmi glykoproteiny zony pellucida (ZP), hyaluronovou kyselinou a heparinem. Afinita n\u011Bkter\u00FDch epididym\u00E1ln\u00EDch protein\u016F k heparinu a hyaluronov\u00E9 kyselin\u011B nazna\u010Duje jejich p\u016Fsoben\u00ED p\u0159i kapacitaci spermi\u00ED ve vejcovodu. Proteinov\u00E9 prou\u017Eky reagovaly na membr\u00E1n\u011B s glykoproteiny ZP. Tento jev by mohl uk\u00E1zat mo\u017Enou \u00FAlohu protein\u016F epididym\u00E1ln\u00ED tekutiny v\u00E1zan\u00FDch na povrch spermi\u00ED p\u0159i epididym\u00E1ln\u00EDm zr\u00E1n\u00ED a tak\u00E9 p\u0159i vazb\u011B spermie s vaj\u00ED\u010Dkem"@cs . "3"^^ . "epididymal fluid proteins; proteinases; binding properties"@en . . . . "The ability of some epididymal proteins to associate with spermatozoa suggests their role in sperm maturation and/or in consequential phases of the fertilization process. By size exclusion chromatography, RP HPLC and SDS-PAGE we separated proteins from boar epididymal fluid. By the zymography method, we detected serine- and metallo-proteinases. Epididymal fluid proteins were tested with biotinylated glycoproteins of zona pellucida (ZP), hyaluronic acid, and heparin on nitrocellulose membrane after Western blot. The affinity of some epididymal proteins with heparin and hyaluronic acid suggests their role in sperm capacitation in the oviduct. Protein bands interacted with ZP glycoproteins on the blot. This phenomenon might indicate a possible role of epididymal fluid proteins bound to the sperm surface in epididymal maturation and in the sperm-egg binding" . "Madison" . . . "2"^^ . .