"US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Demethylation of host-cell DNA at the site of avian retrovirus integration." . "6"^^ . . . . . . . "Demethylation of host-cell DNA at the site of avian retrovirus integration." . "Bla\u017Ekov\u00E1, Jana" . "0"^^ . . "Z(AV0Z5052915)" . "Demethylation of host-cell DNA at the site of avian retrovirus integration."@en . "0"^^ . . . "4"^^ . "0006-291X" . "311" . . "Demethylation of host-cell DNA at the site of avian retrovirus integration."@en . "Walter, J." . "The transcriptional activity of an integrated retroviral copy strongly depends on the adjacent host-cell DNA at the site of integration. Transcribed DNA loci as well as cis-acting sequences like enhancers or CpG islands usually permit expression of nearby integrated proviruses. In contrast, proviruses residing close to cellular silencers tend to transcriptional silencing and CpG methylation. Little is known, however, about the influence of provirus integration on the target sequence in the host genome. Here, we report interesting features of a simplified Rous sarcoma virus integrated into a non-transcribed hypermethylated DNA sequence in the Syrian hamster genome. After integration, CpG methylation of this sequence has been lost almost completely and hypomethylated DNA permits proviral transcription and hamster cell transformation by the proviral v-src oncogene. This, however, is not a stable state, and non-transformed revertants bearing transcriptionally silenced proviruses segregate with a high rate" . . . "RIV/68378050:_____/03:23033178" . . "H\u00E1jkov\u00E1, P." . "DNA methylation and demethylation; integration of retroviruses; gene silencing"@en . "Biochemical and biophysical research vommunications" . "The transcriptional activity of an integrated retroviral copy strongly depends on the adjacent host-cell DNA at the site of integration. Transcribed DNA loci as well as cis-acting sequences like enhancers or CpG islands usually permit expression of nearby integrated proviruses. In contrast, proviruses residing close to cellular silencers tend to transcriptional silencing and CpG methylation. Little is known, however, about the influence of provirus integration on the target sequence in the host genome. Here, we report interesting features of a simplified Rous sarcoma virus integrated into a non-transcribed hypermethylated DNA sequence in the Syrian hamster genome. After integration, CpG methylation of this sequence has been lost almost completely and hypomethylated DNA permits proviral transcription and hamster cell transformation by the proviral v-src oncogene. This, however, is not a stable state, and non-transformed revertants bearing transcriptionally silenced proviruses segregate with a high rate"@en . "Hejnar, Ji\u0159\u00ED" . "Elleder, Daniel" . "641;648" . . . "[39E72E14F13B]" . . "8"^^ . . "2003" . "RIV/68378050:_____/03:23033178!RIV/2004/AV0/A23004/N" . "603029" . "Svoboda, Jan" .