. "The nonclassical class I MHC molecule HLA-G is selectively expressed on extravillous cytotrophoblast cells. HLA-G can inhibit the killing mediated by NK cells via interaction with the inhibitory NK cell receptor LIR-1. Comparison of the sequence of the HLA-G molecule to other class I MHC proteins revealed two unique cysteine residues 42 and 147. Mutating these cysteine residues resulted in a dramatic decrease in LIR-1 Ig binding. The mutated HLA-G transfectants were less effective in the inhibition of NK killing. The involvement of the cysteine residues in the formation of HLA-G protein oligomers was demonstrated. The cysteine residue 42 is critical for the expression of such complexes. These oligomers, unique among the class I MHC proteins, probably bind to LIR-1 with increased avidity, resulting in an enhanced inhibitory function of LIR-1 and an impaired killing function of NK cells." . "Hanna, J." . "[C15DE16BFA21]" . "Mandelboim, O." . . "171" . "RIV/68378050:_____/03:23033071" . "Journal of Immunology" . "RIV/68378050:_____/03:23033071!RIV/2004/AV0/A23004/N" . "1343;1351" . "P(LN00A026), Z(AV0Z5052915)" . "The nonclassical class I MHC molecule HLA-G is selectively expressed on extravillous cytotrophoblast cells. HLA-G can inhibit the killing mediated by NK cells via interaction with the inhibitory NK cell receptor LIR-1. Comparison of the sequence of the HLA-G molecule to other class I MHC proteins revealed two unique cysteine residues 42 and 147. Mutating these cysteine residues resulted in a dramatic decrease in LIR-1 Ig binding. The mutated HLA-G transfectants were less effective in the inhibition of NK killing. The involvement of the cysteine residues in the formation of HLA-G protein oligomers was demonstrated. The cysteine residue 42 is critical for the expression of such complexes. These oligomers, unique among the class I MHC proteins, probably bind to LIR-1 with increased avidity, resulting in an enhanced inhibitory function of LIR-1 and an impaired killing function of NK cells."@en . "HLA-G; LIR-1; NK cells"@en . . . "601853" . "Complexes of HLA-G protein on the cell surface are important for leukocyte Ig-like receptor-1 function." . "Achdout, H." . . "Ho\u0159ej\u0161\u00ED, V\u00E1clav" . "Baniyash, M." . "Gonen-Gross, T." . "Complexes of HLA-G protein on the cell surface are important for leukocyte Ig-like receptor-1 function." . . "Levy, O." . . . "Complexes of HLA-G protein on the cell surface are important for leukocyte Ig-like receptor-1 function."@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "0022-1767" . "12"^^ . . . . "Complexes of HLA-G protein on the cell surface are important for leukocyte Ig-like receptor-1 function."@en . . "Goldman-Wohl, D." . . "Markel, G." . "0"^^ . "3" . "0"^^ . . . "1"^^ . "4"^^ . "Yagel, S." . "Gazit, R." . "Mizrahi, S." . . .