. "0"^^ . "Journal of Biological Chemistry" . "601575" . "0"^^ . . . . . "5"^^ . "Tasken, K." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Treatment of cells with the cAMP-elevating agent prostaglandin E2 (PGE2) augmented the level of Cbp/PAG phosphorylation with a concomitant increase in amounts of Csk bound to Cbp/PAG. While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE2 reduced Csk dissociation upon TCR triggering. This correlated with lowered TCR-induced phosphorylation of CD3 zeta-chain and linker for activation of T cells. Moreover, competition of endogenous Csk from lipid rafts abolished PGE2-mediated inhibition of TCR-induced zeta-chain phosphorylation and activation of NFAT and AP-1. Finally, raft-associated Csk already activated via Cbp/PAG binding, gained additional increase in phosphotransferase activity upon protein kinase A-mediated phosphorylation of Csk. It is proposed that cAMP regulates Csk via both spatial and enzymatic mechanisms, thereby inhibiting signaling through the TCR." . "RIV/68378050:_____/03:23033070!RIV/2004/AV0/A23004/N" . . "278" . "[844BF2A1950E]" . "Treatment of cells with the cAMP-elevating agent prostaglandin E2 (PGE2) augmented the level of Cbp/PAG phosphorylation with a concomitant increase in amounts of Csk bound to Cbp/PAG. While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE2 reduced Csk dissociation upon TCR triggering. This correlated with lowered TCR-induced phosphorylation of CD3 zeta-chain and linker for activation of T cells. Moreover, competition of endogenous Csk from lipid rafts abolished PGE2-mediated inhibition of TCR-induced zeta-chain phosphorylation and activation of NFAT and AP-1. Finally, raft-associated Csk already activated via Cbp/PAG binding, gained additional increase in phosphotransferase activity upon protein kinase A-mediated phosphorylation of Csk. It is proposed that cAMP regulates Csk via both spatial and enzymatic mechanisms, thereby inhibiting signaling through the TCR."@en . . "Myklebust, S." . . . . . "Ho\u0159ej\u0161\u00ED, V\u00E1clav" . "P(LN00A026), Z(AV0Z5052915)" . "20" . "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." . "Abrahamsen, H." . "RIV/68378050:_____/03:23033070" . "Vang, T." . "cAMP; Csk; T cell activation"@en . "0021-9258" . . "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." . "17597;17600" . "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling."@en . . . . "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling."@en . . "1"^^ . . "4"^^ .