. . "Naumann, P. W." . . "P(GA301/01/0041), Z(AV0Z5052915)" . "Valenta, Tom\u00E1\u0161" . "611238" . "Iron deprivation induces apoptosis in some sensitive cultured tumour cells, while other cells are resistant. In order to elucidate the mechanisms involved in apoptosis induction by iron deprivation, we studied the expression of p53 and the expression of selected p53-regulated genes. To discriminate between changes coupled only with iron deprivation and changes involved in apoptosis induction by iron deprivation, we compared the expression of the genes in sensitive (human Raji, mouse 38C13) versus resistant (human HeLa, mouse EL4) cells under iron deprivation. Iron deprivation was achieved by incubation in a defined iron-free medium. The level of p53 mRNA decreased significantly under iron deprivation in sensitive cells, but it did not change in resistant cells. On the contrary, the level of the p53 protein under iron deprivation was slightly increased in sensitive cells while it was not changed in resistant cells. The activity of p53 was assessed by the expression of selected p53-re..."@en . "Iron deprivation induces apoptosis independently of p53 in human and murine tumour cells"@en . "Iron deprivation induces apoptosis independently of p53 in human and murine tumour cells" . . "Iron deprivation induces apoptosis in some sensitive cultured tumour cells, while other cells are resistant. In order to elucidate the mechanisms involved in apoptosis induction by iron deprivation, we studied the expression of p53 and the expression of selected p53-regulated genes. To discriminate between changes coupled only with iron deprivation and changes involved in apoptosis induction by iron deprivation, we compared the expression of the genes in sensitive (human Raji, mouse 38C13) versus resistant (human HeLa, mouse EL4) cells under iron deprivation. Iron deprivation was achieved by incubation in a defined iron-free medium. The level of p53 mRNA decreased significantly under iron deprivation in sensitive cells, but it did not change in resistant cells. On the contrary, the level of the p53 protein under iron deprivation was slightly increased in sensitive cells while it was not changed in resistant cells. The activity of p53 was assessed by the expression of selected p53-re..." . "Cell Proliferation" . "Nedostatek \u017Eeleza vyvol\u00E1v\u00E1 apopt\u00F3zu nez\u00E1visle na p53 v lidsk\u00FDch a my\u0161\u00EDch n\u00E1dorov\u00FDch bu\u0148k\u00E1ch"@cs . "Iron deprivation induces apoptosis independently of p53 in human and murine tumour cells"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "6"^^ . . "iron deprivation; p53; apoptosis induction"@en . "RIV/68378050:_____/03:00105357!RIV/2005/GA0/A23005/N" . . . "199;213" . . "Iron deprivation induces apoptosis independently of p53 in human and murine tumour cells" . . "36" . . "4" . "Nedostatek \u017Eeleza vyvol\u00E1v\u00E1 apopt\u00F3zu nez\u00E1visle na p53 v lidsk\u00FDch a my\u0161\u00EDch n\u00E1dorov\u00FDch bu\u0148k\u00E1ch"@cs . "Nedostatek \u017Eeleza vyvol\u00E1v\u00E1 v n\u011Bkter\u00FDch citliv\u00FDch kultivovan\u00FDch n\u00E1dorov\u00FDch bu\u0148k\u00E1ch apopt\u00F3zu, zat\u00EDmco jin\u00E9 bu\u0148ky odol\u00E1vaj\u00ED. Abychom objasnili mechanismy pod\u00EDlej\u00EDc\u00ED se na vyvol\u00E1n\u00ED apopt\u00F3zy nedostatkem \u017Eeleza, studovali jsme expresi p53 a expresi vybran\u00FDch gen\u016F regulovan\u00FDch p53. K odli\u0161en\u00ED zm\u011Bn spojen\u00FDch pouze s nedostatkem \u017Eeleza od zm\u011Bn spojen\u00FDch s vyvol\u00E1n\u00EDm apopt\u00F3zy nedostatkem \u017Eeleza jsme srovnali expresi gen\u016F v citliv\u00FDch (lidsk\u00E9 Raji, my\u0161\u00ED 38C13) a odoln\u00FDch (lidsk\u00E9 HeLa, my\u0161\u00ED EL4) bu\u0148k\u00E1ch p\u0159i nedostatku \u017Eeleza. Nedostatek \u017Eeleza byl vyvol\u00E1n inkubac\u00ED v definovan\u00E9m m\u00E9diu zbaven\u00E9m \u017Eeleza. Hladina p53 mRNA se p\u0159i nedostatku \u017Eeleza v citliv\u00FDch bu\u0148k\u00E1ch v\u00FDrazn\u011B sn\u00ED\u017Eila, ale v odoln\u00FDch bu\u0148k\u00E1ch se nezm\u011Bnila. Naopak hladina proteinu p53 byla p\u0159i nedostatku \u017Eeleza v citliv\u00FDch bu\u0148k\u00E1ch m\u00EDrn\u011B zv\u00FD\u0161en\u00E1 a v odoln\u00FDch bu\u0148k\u00E1ch beze zm\u011Bn. Aktivita p53 byla hodnocena expres\u00ED vybran\u00FDch c\u00EDl\u016F regulovan\u00FDch p53, tj. gen\u016F p21WAF1/CIP1, mdm2, bcl-2 a bax. Nezjistili jsme \u017E\u00E1dnou v\u00FDraznou zm\u011Bnu v hladin\u00E1ch mRNA ..."@cs . . . "15"^^ . "[B55E0C6024F6]" . "Pol\u00E1k, J." . . "4"^^ . . . "Kov\u00E1\u0159, Jan" . "RIV/68378050:_____/03:00105357" . "Truksa, Jaroslav" . . . "Ehrlichov\u00E1, Marie" . . .