. "11"^^ . "Kov\u00E1\u0159, Jan" . "We studied the effects of thiol availability on apoptosis induction in B-cell lymphoma 38C13, T-cell lymphoma EL4, and also other cells. Compounds with a free SH group are required for survival and growth of 38C13 cells but not of EL4 cells. Thiol deprivation (2-mercaptoethanol concentrations about 0.3 microM and lower) induced apoptosis in 38C13 cells. On the other hand, thiol excess (2-mercaptoethanol concentrations higher than 300 microM) induced apoptosis in 38C13 cells and EL4 cells as well as in other cells (e.g. Raji, HeLa). L-cystine and non-thiol antioxidant ascorbic acid were unable to support survival of 38C13 cells. Ascorbic acid induced cell death at concentrations higher than 600 microM. Thiol cross-linking compound diamide (100 microM and higher) abrogated the survival-supporting effect of 2-mercaptoethanol (50 microM). Apoptosis induction by thiol deprivation and by thiol excess was not directly related to a specific significant change in the p53 level or p53 activation. Apoptosis indu" . . "Apoptosis induction in lymphoma cells: thiol deprivation versus thiol excess." . . . . . . "Truksa, Jaroslav" . "0"^^ . . "0"^^ . "RIV/68378050:_____/02:23033134!RIV/2004/GA0/A23004/N" . . "We studied the effects of thiol availability on apoptosis induction in B-cell lymphoma 38C13, T-cell lymphoma EL4, and also other cells. Compounds with a free SH group are required for survival and growth of 38C13 cells but not of EL4 cells. Thiol deprivation (2-mercaptoethanol concentrations about 0.3 microM and lower) induced apoptosis in 38C13 cells. On the other hand, thiol excess (2-mercaptoethanol concentrations higher than 300 microM) induced apoptosis in 38C13 cells and EL4 cells as well as in other cells (e.g. Raji, HeLa). L-cystine and non-thiol antioxidant ascorbic acid were unable to support survival of 38C13 cells. Ascorbic acid induced cell death at concentrations higher than 600 microM. Thiol cross-linking compound diamide (100 microM and higher) abrogated the survival-supporting effect of 2-mercaptoethanol (50 microM). Apoptosis induction by thiol deprivation and by thiol excess was not directly related to a specific significant change in the p53 level or p53 activation. Apoptosis indu"@en . . . . . . . "Apoptosis induction in lymphoma cells: thiol deprivation versus thiol excess."@en . . "5"^^ . "Valenta, Tom\u00E1\u0161" . "Apoptosis induction in lymphoma cells: thiol deprivation versus thiol excess." . "48" . "2" . . "58;68" . "Folia Biologica" . "[9488BF8482B9]" . "5"^^ . . "P(GA301/01/0041), P(GA301/97/1029), Z(AV0Z5052915)" . . "\u0160t\u00FDbrov\u00E1, Hana" . "Apoptosis induction; thiol deprivation; thiol excess"@en . "Apoptosis induction in lymphoma cells: thiol deprivation versus thiol excess."@en . "Sp\u011Bv\u00E1kov\u00E1, Kate\u0159ina" . "0015-5500" . "CZ - \u010Cesk\u00E1 republika" . . . . "638730" . "RIV/68378050:_____/02:23033134" .