. "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor." . . . . "We prepared monoclonal antibodies against lipid rafts from the rat basophilic leukemia cell line, RBL-2H3. One of the antibodies was found to recognize a novel glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of 250 amino acids, designated TEC-21, containing a cysteine-rich domain homologous to those found in the urokinase plasminogen activator receptor/Ly6/snake neurotoxin family. The TEC-21 is abundant on the surface of RBL-2H3 cells (>106 molecules/cell) but is absent in numerous rat tissues except for testes. Aggregation of TEC-21 on RBL-2H3 cells induced a rapid increase in tyrosine phosphorylation of several substrates including Syk kinase and LAT adaptor, calcium flux, and release of secretory components. TEC-21-induced activation events were also observed in Fce receptorI-negative mutants of RBL-2H3 cells. The combined data suggest that TEC-21 is a novel lipid raft component of RBL-2H3 cells whose aggregation induces activation independently of Fce receptorI." . "Dr\u00E1berov\u00E1, Lubica" . "2" . "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor."@en . "14" . . . . "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor." . . "[97C5D0468765]" . "We prepared monoclonal antibodies against lipid rafts from the rat basophilic leukemia cell line, RBL-2H3. One of the antibodies was found to recognize a novel glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of 250 amino acids, designated TEC-21, containing a cysteine-rich domain homologous to those found in the urokinase plasminogen activator receptor/Ly6/snake neurotoxin family. The TEC-21 is abundant on the surface of RBL-2H3 cells (>106 molecules/cell) but is absent in numerous rat tissues except for testes. Aggregation of TEC-21 on RBL-2H3 cells induced a rapid increase in tyrosine phosphorylation of several substrates including Syk kinase and LAT adaptor, calcium flux, and release of secretory components. TEC-21-induced activation events were also observed in Fce receptorI-negative mutants of RBL-2H3 cells. The combined data suggest that TEC-21 is a novel lipid raft component of RBL-2H3 cells whose aggregation induces activation independently of Fce receptorI."@en . . . . "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor."@en . "1460-2377" . "3"^^ . . . "3"^^ . . "0"^^ . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "H\u00E1lov\u00E1, Ivana" . . "International Immunology" . "0"^^ . . "RIV/68378050:_____/02:23033123!RIV/2004/GA0/A23004/N" . . . "Dr\u00E1ber, Petr" . "637134" . "RIV/68378050:_____/02:23033123" . . "213;223" . "11"^^ . "mast cell; plasma membrane; IgE"@en . . . "P(GA204/00/0204), P(GA310/00/0205), P(GV312/96/K205), P(IAA5052005), P(IAA7052006), P(LN00A026), Z(AV0Z5052915)" . . .