. . "13"^^ . . "Kraus, Marek" . "RIV/68378050:_____/02:00105273!RIV/2005/AV0/A23005/N" . "Opa\u010Dn\u00FD efekt indomethacinu na imunosupresivn\u00ED aktivitu kan\u010D\u00ED semenn\u00E9 imunosupresivn\u00ED frakce"@cs . . "71" . . "NL - Nizozemsko" . . "8"^^ . "P(GA303/01/0615), P(GV524/96/K162), P(KSK5011112), P(NI6883), Z(AV0Z5045916), Z(AV0Z5052915)" . . "7"^^ . . "M\u011B\u0159en\u00EDm inkorporace bromodeoxyuridinu do replikuj\u00EDc\u00ED se DNA jsme zjistili, \u017Ee imunosupresivn\u00ED frakce kan\u010D\u00ED semenn\u00E9 plazmy (ISF) potla\u010Duje proliferaci lymfocyt\u016F stimulovanou mitogeny: p\u0159edb\u011B\u017En\u00E9 p\u016Fsoben\u00ED monoklon\u00E1ln\u00ED protil\u00E1tkou proti ISF \u010Di indomethacinem \u00FA\u010Dinek ISF zvr\u00E1tilo. Krom\u011B toho jsme sledovali schopnost indomethacinu \u010Di monoklon\u00E1ln\u00ED protil\u00E1tky proti ISF zvr\u00E1tit potla\u010Den\u00ED produkce protil\u00E1tek vyvolan\u00E9 ISF m\u011B\u0159en\u00EDm titru protil\u00E1tek v krevn\u00EDm s\u00E9ru my\u0161\u00ED imunizovan\u00FDch %22keyhole limpet%22 hemocyaninem pomoc\u00ED ELISA. V ned\u00E1vn\u00E9 dob\u011B jsme popsali ISF jako komplex hlavn\u00EDch semenn\u00FDch glykoprotein\u016F PSP I a PSP II. PSP II je slo\u017Eka odpov\u011Bdn\u00E1 za imunosupresivn\u00ED schopnosti tohoto komplexu. Abychom zjistili, zda je imunosupresivn\u00ED \u00FA\u010Dinek ISF spojen s jeho proteinovou \u010Di sacharidovou \u010D\u00E1st\u00ED, zkoumali jsme u deglykosylovan\u00E9ho PSP II jeho antiproliferativn\u00ED \u00FA\u010Dinek na my\u0161\u00ED lymfocyty stimulovan\u00E9 mitogeny. V\u00FDsledky ukazuj\u00ED, \u017Ee deglykosylace PSP II nem\u011Bla vliv na jeho antiproliferativn\u00ED aktivitu. Z toho vypl\u00FDv\u00E1, \u017Ee imuno..."@cs . "Animal Reproduction Science" . . "Dost\u00E1l, Jarom\u00EDr" . "Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction" . "By measuring bromodeoxyuridine incorporation into replicated DNA, we found that immunosuppressive fraction of boar seminal plasma (ISF) inhibited mitogen-stimulated lymphocyte proliferation; pretreatment with monoclonal antibody to ISF or indomethacin reversed the ISF effect. Besides, we followed the ability of indomethacin or monoclonal antibody to ISF to reverse ISF-induced suppression of antibody production by measuring antibody titers in the blood sera of mice immunized with keyhole limpet hemocyanin using ELISA. Recently we have identified ISF as a complex of the major seminal glycoproteins PSP I and PSP II. PSP II is the part that is responsible for immunosuppressive properties of the complex. To learn whether the ISF immunosuppressive effect is associated with its protein or saccharide part, we examined the deglycosylated PSP II for its antiproliferative effect on mitogen-stimulated mouse lymphocytes. The results suggest that deglycosylation of PSP II did not affect its antiproliferative ac..."@en . . . "\u017Delezn\u00E1, Blanka" . . . "111;123" . "RIV/68378050:_____/02:00105273" . "Hol\u00E1\u0148, Vladim\u00EDr" . . "Opa\u010Dn\u00FD efekt indomethacinu na imunosupresivn\u00ED aktivitu kan\u010D\u00ED semenn\u00E9 imunosupresivn\u00ED frakce"@cs . "Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction"@en . "Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction" . "0378-4320" . . . "662337" . . . "Zaj\u00EDcov\u00E1, Alena" . . . "Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction"@en . . . . "By measuring bromodeoxyuridine incorporation into replicated DNA, we found that immunosuppressive fraction of boar seminal plasma (ISF) inhibited mitogen-stimulated lymphocyte proliferation; pretreatment with monoclonal antibody to ISF or indomethacin reversed the ISF effect. Besides, we followed the ability of indomethacin or monoclonal antibody to ISF to reverse ISF-induced suppression of antibody production by measuring antibody titers in the blood sera of mice immunized with keyhole limpet hemocyanin using ELISA. Recently we have identified ISF as a complex of the major seminal glycoproteins PSP I and PSP II. PSP II is the part that is responsible for immunosuppressive properties of the complex. To learn whether the ISF immunosuppressive effect is associated with its protein or saccharide part, we examined the deglycosylated PSP II for its antiproliferative effect on mitogen-stimulated mouse lymphocytes. The results suggest that deglycosylation of PSP II did not affect its antiproliferative ac..." . . "Jon\u00E1kov\u00E1, V\u011Bra" . "Veselsk\u00FD, Leopold" . "[B2C3DE02D7DC]" . . . "Indomethacin; Antibody response; Seminal immunosuppressive fraction"@en . . "P\u011Bknicov\u00E1, Jana" .