"RIV/68378050:_____/01:00105389!RIV/2005/AV0/A23005/N" . "RIV/68378050:_____/01:00105389" . "Expression of human erythropoietin gene in the mammary gland of a transgenic mouse"@en . . . "Folia Biologica" . "Miku\u0161, Tom\u00E1\u0161" . "6"^^ . "Exprese genu pro lidsk\u00FD erytropoietin v ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze transgenn\u00ED my\u0161i"@cs . . "Expression of human erythropoietin gene in the mammary gland of a transgenic mouse" . "[7F32C198CC16]" . "Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5\u017D-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5\u017D-untranslated part of the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro..."@en . "3"^^ . "6" . "0015-5500" . "47" . "Expression of human erythropoietin gene in the mammary gland of a transgenic mouse"@en . . . "Expression of human erythropoietin gene in the mammary gland of a transgenic mouse" . "Landa, Vladim\u00EDr" . . . "Mal\u00FD, Petr" . "Exprese genu pro lidsk\u00FD erytropoietin v ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze transgenn\u00ED my\u0161i"@cs . "187;195" . "Popl\u0161tein, M." . . "CZ - \u010Cesk\u00E1 republika" . . "680084" . . "erythropoietin, mammary gland, transgenic mouse"@en . "Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5\u017D-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5\u017D-untranslated part of the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro..." . . . "Kysel\u00FD protein syrov\u00E1tky, WAP, se pova\u017Euje za nejd\u016Fle\u017Eit\u011Bj\u0161\u00ED ml\u00E9\u010Dn\u00FD protein exprimovan\u00FD v b\u0159ez\u00EDch \u010Di koj\u00EDc\u00EDch samic u \u0159ady sav\u010D\u00EDch druh\u016F. Ned\u00E1vno bylo prok\u00E1z\u00E1no, \u017Ee 5\u00B4-netranslatovan\u00E1 oblast kr\u00E1li\u010D\u00EDho genu pro WAP (rWAP) o 6,3 kb m\u016F\u017Ee \u0159\u00EDdit t\u00E9m\u011B\u0159 zcela omezit expresi transgenu v ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze trangenn\u00EDho zv\u00ED\u0159ete. Klonovali jsme fragment genomu obsahuj\u00EDc\u00ED lokus genu rWAP z f\u00E1gov\u00E9 genomov\u00E9 knihovny ?DASHII a vyu\u017Eili jsme 8,5 kb dlouhou 5\u00B4-netranslatovanou \u010D\u00E1st genu rWAP k c\u00EDlen\u00ED exprese lidsk\u00E9ho genu pro erytropoietin (hEPO), klonovan\u00E9ho z f\u00E1gov\u00E9 genomov\u00E9 knihovny ?FIXII, do ml\u00E9\u010Dn\u00E9 \u017El\u00E1zy my\u0161i. Vektory obsahuj\u00EDc\u00ED gen pro hEPO \u010Di hybridn\u00ED genov\u00FD konstrukt rWAP-hEPO byly injikov\u00E1ny do my\u0161\u00EDch vaj\u00ED\u010Dek a pomoc\u00ED PCR a Southernova p\u0159enosu bylo mezi 168 testovan\u00FDmi potomky identifikov\u00E1no 12 transgenn\u00EDch zv\u00ED\u0159at. Transgenn\u00ED my\u0161\u00ED byly \u017Eivotaschopn\u00E9, plodn\u00E9 a norm\u00E1ln\u011B se vyv\u00EDjely. Rekombinantn\u00ED lidsk\u00FD erytropoietin byl produkov\u00E1n v ml\u00E9ce transgenn\u00ED my\u0161\u00ED samice na \u00FArovni sekrece 5,345 mIU/ml, jak by..."@cs . . "Trefil, P." . . . . "Lidick\u00FD, J." . "Z(AV0Z5052915)" . "9"^^ . .