"fluorecence dynamics; transient binding; diffusion"@en . . . "Buzgo, Matej" . "Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol\u2013chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules." . "Luk\u00E1\u0161, D." . "1742-7061" . "000297436500012" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "Amler, Ev\u017Een" . . . "Huranov\u00E1, Martina" . . . . "2"^^ . "Humpol\u00ED\u010Dkov\u00E1, Jana" . "Mach\u00E1\u0148, Radek" . . "Hof, Martin" . . "Acta Biomaterialia" . . . . "[50FF43BA0143]" . "8"^^ . "Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds"@en . "9"^^ . . "10.1016/j.actbio.2011.07.012" . "Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds" . "Norris, S. C. P." . . "RIV/68378041:_____/11:00368065!RIV12-AV0-68378041" . . . "7" . . . "Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds"@en . . . "P(GA202/09/1151), P(GAP304/10/1307), P(IAA500390702), P(LC06063), Z(AV0Z40400503), Z(AV0Z50390512), Z(AV0Z50390703), Z(AV0Z50520514)" . "Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol\u2013chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules."@en . "Buzgo, Matej" . "225727" . "12" . . . "Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds" . "RIV/68378041:_____/11:00368065" .