. . . . . "2"^^ . . "104701" . "Matalov\u00E1, Eva" . "3"^^ . "1213-7103" . . "Chemick\u00E9 Listy. Ro\u010D. 107, Issue s3." . . "2"^^ . . "Brno" . "caspase-3; single stem cell; miniaturization"@en . . . "Senzitive method of Caspase-3 detection in single stem cell" . . "Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10\u201315 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime." . "2013-11-12+01:00"^^ . "RIV/68081715:_____/13:00420849" . "Senzitive method of Caspase-3 detection in single stem cell"@en . "Senzitive method of Caspase-3 detection in single stem cell" . . "[85C6F701EFA0]" . "RIV/68081715:_____/13:00420849!RIV14-MSM-68081715" . . "Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10\u201315 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime."@en . . . . . "Senzitive method of Caspase-3 detection in single stem cell"@en . "\u010Cesk\u00E1 spole\u010Dnost chemick\u00E1" . "I, P(EE2.3.20.0182), P(GAP206/11/2377)" . "Praha" . . "Adamov\u00E1, Eva" . . "Klep\u00E1rn\u00EDk, Karel" . "000328730800002" .