"000302960100013" . "1" . . "HISTONE DEACETYLASE INHIBITOR; ENHANCES RADIATION SENSITIVITY; TRANSFORMED-CELLS"@en . . "Maz\u00E1nkov\u00E1, N." . . "\u0158ez\u00E1\u010Dov\u00E1, M." . "176918" . "0026-8933" . . "I" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "[69AFF8203FD0]" . "10.1134/S0026893312010141" . . "46" . "8"^^ . . "V\u00E1vrov\u00E1, J." . "Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells" . . . . "Kn\u00ED\u017Eek, J." . "7"^^ . . "Molecular Biology" . "Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells"@en . "The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two-millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4 h post-irradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA-treated cells."@en . "Muthna, D." . . . "RIV/68081707:_____/12:00389863" . "Luk\u00E1\u0161ov\u00E1, Emilie" . "Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells" . "1"^^ . "The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two-millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4 h post-irradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA-treated cells." . . . "K\u00F6hlerov\u00E1, R." . "Tich\u00FD, A." . . "RIV/68081707:_____/12:00389863!RIV13-AV0-68081707" . "Valproic Acid Decreases the Reparation Capacity of Irradiated MOLT-4 Cells"@en .