. . . . . . . "Kova\u0159\u00EDk, Ale\u0161" . . . "Maty\u00E1\u0161ek, Roman" . "10.1111/j.1095-8339.2012.01280.x" . "3" . . . "RIV/68081707:_____/12:00389788!RIV13-AV0-68081707" . "0024-4074" . "120512" . "13"^^ . "A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous"@en . "170" . . . . "Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats." . "Botanical Journal of the Linnean Society" . . "5"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "cell biology; cell cycle; evolution"@en . . "A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous" . "Sou\u010Dkov\u00E1, Kamila" . "000310079500007" . "A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous"@en . "Leitch, A. R." . "RIV/68081707:_____/12:00389788" . "Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats."@en . . . "Lim, K. Y." . "3"^^ . "[8DA0CCE71089]" . "A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous" . . "I, P(GAP501/10/0208)" .