"6"^^ . "10.1039/c2sc20404e" . "9" . "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA\u2013protein interactions" . . "10"^^ . . . "RIV/68081707:_____/12:00382144!RIV13-AV0-68081707" . . "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA\u2013protein interactions"@en . "I, P(GA203/09/0317), P(GBP206/12/G151)" . "146252" . "3" . . "Hocek, Michal" . "Riedl, Jan" . "2041-6520" . . . . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "Solvatochromic fluorescent 4-aminophthalimide and 4-(N,N-dimethylamino)phthalimide were attached covalently to 2'-deoxycytidine or -adenosine via non-conjugated propargyl linker by Sonogashira cross-coupling reactions. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated to oligonucleotides by DNA polymerases. API-labelled DNA was used for detection of DNA protein interactions with p53 protein or single strand binding protein. Both proteins changed the polarity around the fluorophore and increased the intensity of API fluorescence." . "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA\u2013protein interactions" . "Ors\u00E1g, Petr" . . . . . "Solvatochromic fluorescent 4-aminophthalimide and 4-(N,N-dimethylamino)phthalimide were attached covalently to 2'-deoxycytidine or -adenosine via non-conjugated propargyl linker by Sonogashira cross-coupling reactions. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated to oligonucleotides by DNA polymerases. API-labelled DNA was used for detection of DNA protein interactions with p53 protein or single strand binding protein. Both proteins changed the polarity around the fluorophore and increased the intensity of API fluorescence."@en . "[B6E58584644F]" . "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA\u2013protein interactions"@en . "RIV/68081707:_____/12:00382144" . "2"^^ . . . "DNA; oligonucleotides; polymerase; phthalimide; nucleotides; fluorescent labeling"@en . . . . "Ernsting, N. P." . . . "Fojta, Miroslav" . "Chemical Science" . "Pohl, Radek" . "000306931300019" .