"1933;1946" . . . "14"^^ . "0305-1048" . "The genomic HDV ribozyme utilizes a previously unnoticed U-turn motif to accomplish fast site-specific catalysis"@en . . . "RIV/68081707:_____/07:00081998!RIV07-AV0-68081707" . . "The genomic HDV ribozyme utilizes a previously unnoticed U-turn motif to accomplish fast site-specific catalysis" . "The genome of the human hepatitis delta virus (HDV) harbors a self-cleavage catalytic RNA motif, the genomic HDV ribozyme, whose crystal structure shows the dangling nucleotides 5' of the cleavage site projecting away from the catalytic core. This 5' sequence contains a clinically conserved U-1 that we find essential for fast cleavage. Molecular dynamics simulations demonstrate that a U-1 forms the robust kink around the scissile phosphate, exposing it to the catalytic C75 in a previously unnoticed U-turn motif found also, for example, in the hammerhead ribozyme and tRNAs. We find that the common structural U-turn motif serves distinct functions in the HDV and hammerhead ribozymes." . . "The genomic HDV ribozyme utilizes a previously unnoticed U-turn motif to accomplish fast site-specific catalysis" . "2"^^ . "Genom lidsk\u00E9ho hepatitis delta viru (HDV) obsahuje samo\u0161t\u011Bpic\u00ED se katalytick\u00FD RNA motiv, genomick\u00FD HDV ribozyme, jeho\u017E krystalov\u00E1 struktura ukazuje voln\u00E9 nukleotidy 5' m\u00EDsta \u0161t\u011Bpen\u00ED vy\u010Dn\u00EDvaj\u00EDc\u00ED mimo katalytick\u00E9ho j\u00E1dra. Tato 5' sekvence obsahuje klinicky konzervovan\u00FD U-1, kter\u00FD je, jak jsme zjistili, esenci\u00E1ln\u00ED pro rychl\u00E9 \u0161t\u011Bpen\u00ED. Molekulov\u011B dynamick\u00E9 simulace ukazuj\u00ED, \u017Ee U-1 tvo\u0159\u00ED robustn\u00ED ohyb kolem \u0161t\u011Bpiteln\u00E9ho fosf\u00E1tu, vystavuj\u00EDc ho ku katalytick\u00E9mu C75 v p\u0159edt\u00EDm nepov\u0161imnut\u00E9m U-turn motivu, kter\u00FD se nach\u00E1z\u00ED nap\u0159. v hammerhead ribozymu nebo tRNA. Zjistili jsme, \u017Ee tento b\u011B\u017En\u00FD strukturn\u00ED U-turn motiv m\u00E1 rozd\u00EDln\u00E9 funkce v HDV a hammerhead ribozymu."@cs . "The genome of the human hepatitis delta virus (HDV) harbors a self-cleavage catalytic RNA motif, the genomic HDV ribozyme, whose crystal structure shows the dangling nucleotides 5' of the cleavage site projecting away from the catalytic core. This 5' sequence contains a clinically conserved U-1 that we find essential for fast cleavage. Molecular dynamics simulations demonstrate that a U-1 forms the robust kink around the scissile phosphate, exposing it to the catalytic C75 in a previously unnoticed U-turn motif found also, for example, in the hammerhead ribozyme and tRNAs. We find that the common structural U-turn motif serves distinct functions in the HDV and hammerhead ribozymes."@en . "[523C63B3477D]" . "4"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "hepatitis delta virus; molecular dynamics; self-cleavage"@en . "6" . . "35" . "\u0160ef\u010D\u00EDkov\u00E1, J." . "Walter, N. G." . "423204" . . . . "Krasovsk\u00E1, Maryna V." . . . "RIV/68081707:_____/07:00081998" . "Genomick\u00FD HDV ribozym vyu\u017E\u00EDv\u00E1 p\u0159edt\u00EDm nepov\u0161imnut\u00FD U-turn motiv na rychlou site-specific katal\u00FDzu"@cs . . . . . . . . "P(1QS500040581), P(GA203/05/0009), P(GA203/05/0388), P(IAA400550701), P(LC512), Z(AV0Z40550506), Z(AV0Z50040507)" . . "Genomick\u00FD HDV ribozym vyu\u017E\u00EDv\u00E1 p\u0159edt\u00EDm nepov\u0161imnut\u00FD U-turn motiv na rychlou site-specific katal\u00FDzu"@cs . . "Nucleic Acids Research" . . "\u0160poner, Ji\u0159\u00ED" . "The genomic HDV ribozyme utilizes a previously unnoticed U-turn motif to accomplish fast site-specific catalysis"@en . .