"DNA hybridization on membrane-modified carbon electrodes"@en . "Pale\u010Dek, Emil" . . "Analytical Letters" . . . . "P(1H-PK/42), P(IAA4004402), P(IBS5004355), Z(AV0Z50040507)" . "[2D368542486D]" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Fojta, Miroslav" . "15"^^ . . "Jelen, Franti\u0161ek" . "DNA hybridization on membrane-modified carbon electrodes"@en . "518476" . "7"^^ . . "RIV/68081707:_____/05:00031000" . . "Hybridizace DNA na uhl\u00EDkov\u00FDch elektrod\u00E1ch modifikovan\u00FDch membr\u00E1nou"@cs . "RIV/68081707:_____/05:00031000!RIV06-AV0-68081707" . "6"^^ . "DNA hybridization on membrane-modified carbon electrodes" . "-" . . "0003-2719" . . . . . "DNA hybridization; electrochemical DNA sensor; nitrocellulose membrane"@en . "Hybridizace DNA na uhl\u00EDkov\u00FDch elektrod\u00E1ch modifikovan\u00FDch membr\u00E1nou"@cs . . "Cahov\u00E1-Kucha\u0159\u00EDkov\u00E1, Kate\u0159ina" . "The DNA modified membrane electrode was prepared by casting a mixture of nitrocellulose (NC) with target DNA (tDNA) in organic solvent on glassy carbon electrode (GCE). Unlabelled PCR products (628pb) or synthetic oligodeoxynucleotides (ODNs) were used as tDNA. Biotinylated ODNs were used as hybridization probes. The hybridization events were detected via an enzyme-linked electrochemical assay involving binding of streptavidin-coupled alkaline phosphatase to the biotin labels of the probe bound to tDNA. The enzyme converted the electroinactive substrate (1-naphthyl phosphate) into electroactive product (1-naphthol) that penetrate through the NC membrane to the GCE surface and was subsequently detected using an anodic voltammetric signal." . . "DNA hybridization on membrane-modified carbon electrodes" . "2493;2507" . . . . "Babkina, Sofya" . "Kou\u0159ilov\u00E1, Alena" . . "Havran, Lud\u011Bk" . "38" . "The DNA modified membrane electrode was prepared by casting a mixture of nitrocellulose (NC) with target DNA (tDNA) in organic solvent on glassy carbon electrode (GCE). Unlabelled PCR products (628pb) or synthetic oligodeoxynucleotides (ODNs) were used as tDNA. Biotinylated ODNs were used as hybridization probes. The hybridization events were detected via an enzyme-linked electrochemical assay involving binding of streptavidin-coupled alkaline phosphatase to the biotin labels of the probe bound to tDNA. The enzyme converted the electroinactive substrate (1-naphthyl phosphate) into electroactive product (1-naphthol) that penetrate through the NC membrane to the GCE surface and was subsequently detected using an anodic voltammetric signal."@en . . . . . . "Membr\u00E1nov\u00E1 elektroda modifikovan\u00E1 DNA byla p\u0159ipravena nalit\u00EDm sm\u011Bsi nitrocelul\u00F3zy (NC) s c\u00EDlovou DNA (tDNA) v organick\u00E9m rozpou\u0161t\u011Bdle na elektrodu ze skeln\u00E9ho uhl\u00EDku (GCE). Jako tDNA byly pou\u017Eity nezna\u010Den\u00E9 produkty polymer\u00E1zov\u00E9 \u0159et\u011Bzov\u00E9 reakce nebo syntetick\u00E9 oligonukleotidy (ODN). ODN zna\u010Den\u00E9 biotinem byly pou\u017Eity jako hybridiza\u010Dn\u00ED sondy pro rozpozn\u00E1n\u00ED specifick\u00FDch sekvenc\u00ED. Hybridizace byla detekov\u00E1na pomoc\u00ED imunoenzymov\u00E9 reakce spojen\u00E9 s elektrochemickou detekc\u00ED zahrnuj\u00EDc\u00ED vazbu streptavidunu konjugovan\u00E9ho s alkalickou fosfat\u00E1zou (SALP) na biotinem zna\u010Den\u00E9 hybridiza\u010Dn\u00ED sondy nav\u00E1zan\u00E9 na tDNA. Po hybridizaci sond a nav\u00E1z\u00E1n\u00ED SALP byla elektroda pono\u0159ena do roztoku elektroinaktiovn\u00EDho substr\u00E1tu (1-naftyl fosf\u00E1t). Enzym p\u0159eme\u0148uje tento substr\u00E1t na elektroaktivn\u00ED 1-naftol, kter\u00FD proch\u00E1z\u00ED NC membr\u00E1nou k povrchu GCE kde poskytuje anodick\u00FD sign\u00E1l. Optimalizovan\u00E1 metoda poskytuje dobr\u00E9 rozli\u0161en\u00ED mezi komplement\u00E1rn\u00ED a nespecifickou DNA a to i v p\u0159\u00EDpad\u011B repetitivn\u00EDch sekvenc\u00ED."@cs . .