. . "Vliv monofunk\u010Dn\u00EDch adukt\u016F platnat\u00FDch komplex\u016F na termodynamickou stabilitu a energetiku duplex\u016F DNA"@cs . . "Effects of adducts of [PtCl(NH3)(3)] Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-AGT/ 5'-ACT were prepared and analyzed by differential scanning calorimetry, temperature-dependent ultraviolet absorption and circular dichroism. The unfolding of the platinated duplexes was accompanied by relatively small unfavorable free energy terms. This destabilization was enthalpic in origin. On the other hand, a relatively large reduction of melting temperature (T-m) was observed as a consequence of the monofunctional adduct in the TGT sequence, whereas T-m due to the adduct in the AGT sequence was reduced only slightly."@en . "0006-3495" . . . . "4"^^ . . "P(GA305/02/1552), P(GD204/03/H016), P(IAA5004101), P(KJB5004301), P(OC D20.004), Z(AV0Z50040507)" . "8"^^ . "Brabec, Viktor" . "Biophysical Journal" . "Effects of monofunctional adducts of Platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes" . . . . . . "1207;1214" . "DNA; platinum; cancer"@en . . "519596" . "RIV/68081707:_____/05:00001331!RIV06-AV0-68081707" . "Ka\u0161p\u00E1rkov\u00E1, Jana" . . . . "2" . . "88" . "Byl pops\u00E1n vliv vzniku monofunk\u010Dn\u00EDch adukt\u016F platnat\u00FDch komplex\u016F na termodynamickou stabilitu DNA a d\u016Fsledky vyvolan\u00FDch zm\u011Bn v termodynamick\u00E9 stabilit\u011B modifikovan\u00E9 DNA na jej\u00ED opravu slo\u017Ekami excisn\u00ED nukleotidov\u00E9 opravy."@cs . . "Effects of monofunctional adducts of Platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes"@en . "Effects of monofunctional adducts of Platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes" . "[59DF8926F675]" . . . "Bursov\u00E1, Vendula" . "Hofr, Ctirad" . . "RIV/68081707:_____/05:00001331" . . "Vliv monofunk\u010Dn\u00EDch adukt\u016F platnat\u00FDch komplex\u016F na termodynamickou stabilitu a energetiku duplex\u016F DNA"@cs . "Effects of adducts of [PtCl(NH3)(3)] Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-AGT/ 5'-ACT were prepared and analyzed by differential scanning calorimetry, temperature-dependent ultraviolet absorption and circular dichroism. The unfolding of the platinated duplexes was accompanied by relatively small unfavorable free energy terms. This destabilization was enthalpic in origin. On the other hand, a relatively large reduction of melting temperature (T-m) was observed as a consequence of the monofunctional adduct in the TGT sequence, whereas T-m due to the adduct in the AGT sequence was reduced only slightly." . "Effects of monofunctional adducts of Platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes"@en . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "4"^^ . .