"[379BD88B95B3]" . . "The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum(H) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in %22reduced%22 (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent."@en . . "P(GA305/02/1552), P(GP202/01/D110), P(LZ1K03010), Z(AV0Z50040507)" . "222;229" . "Hofr, Ctirad" . . . "Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)" . . "Biopolymers" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "RIV/68081707:_____/05:00001323" . "0006-3525" . "RIV/68081707:_____/05:00001323!RIV06-AV0-68081707" . "77" . "DNA; interstrand cross-link; transplatin"@en . . . . . "Tepeln\u00E1 stabilita a energetika duplexu DNA 15 p\u00E1r\u016F b\u00E1z\u00ED dlouh\u00E9ho obsahuj\u00EDc\u00EDho mezi\u0159et\u011Bzcov\u00FD m\u016Fstek trans-dimmindichloroplatnat\u00E9ho komplexu"@cs . "2"^^ . . "2"^^ . . "4" . "Byl studov\u00E1n vliv polohy mezi\u0159et\u011Bzcov\u00E9ho m\u016Fstku vytvo\u0159en\u00E9ho trans-dimmindichloroplatnat\u00FDm komplexem (transplatinou) na tepelnou a termodynamickou stabilitu DNA pomoc\u00ED diferen\u010Dn\u00ED skenovac\u00ED kalorimetrie. Bylo zji\u0161t\u011Bno, \u017Ee poloha m\u016Fstku v duplexu DNA moduluje kooperativitu procesu t\u00E1n\u00ED duplexu a n\u00E1sledn\u011B tak\u00E9 tepelnou stabilitu."@cs . . . . "Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)" . . . "Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)"@en . "Tepeln\u00E1 stabilita a energetika duplexu DNA 15 p\u00E1r\u016F b\u00E1z\u00ED dlouh\u00E9ho obsahuj\u00EDc\u00EDho mezi\u0159et\u011Bzcov\u00FD m\u016Fstek trans-dimmindichloroplatnat\u00E9ho komplexu"@cs . "8"^^ . "Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)"@en . "Brabec, Viktor" . "The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum(H) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in %22reduced%22 (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent." . . . . "546689" .