. "Jovin, T. M." . . . . . . "NL - Nizozemsko" . . . . . . . "RIV/68081707:_____/05:00001223" . "5"^^ . "Biophysical Chemistry" . "0301-4622" . "Zachycen\u00ED p53 prost\u0159ednictv\u00EDm filament DNA-protein zobrazen\u00E9 elektronovou"@cs . "[DAD38E2973DF]" . "11"^^ . . . "14" . "Sequestering of p53 into DNA-protein filaments revealed by electron microscopy" . "3"^^ . "Pale\u010Dek, Emil" . "Sequestering of p53 into DNA-protein filaments revealed by electron microscopy"@en . . "Cherny, Dmitry I." . "Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed." . "Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed."@en . "261;271" . "Pale\u010Dek, Jan" . "Sequestering of p53 into DNA-protein filaments revealed by electron microscopy"@en . . "p53; electron microscopy; DNA-protein complex"@en . . . "Pomoc\u00ED elektronov\u00E9 mikroskopie byly analyzov\u00E1ny interakce protein\u016F p53 exprimovan\u00FDch v bakteri\u00EDch, cel\u00E9ho proteinu p53, p53(1-393) a C-termin\u00E1ln\u00EDho fragmentu p53(320-393), s dlouhou dsDNA (p\u0159ibli\u017En\u011B 3000 bp) v line\u00E1rn\u00ED a superhelik\u00E1ln\u00ED form\u011B obsahuj\u00EDc\u00ED \u010Di neobsahuj\u00EDc\u00ED proteinem p53 rozpozn\u00E1vanou sekvenci (p53CON). Hlavn\u00EDm strukturn\u00EDm znakem vytvo\u0159en\u00FDch komplex\u016F byl DNA -protein filament, ve kter\u00E9m jsou duplexy DNA spojeny d\u00EDky nav\u00E1zan\u00E9mu tetrametru proteinu. V pr\u00E1ci byly diskutov\u00E1ny mo\u017En\u00E9 d\u016Fsledky zachycen\u00ED p53 prost\u0159ednictv\u00EDm tvorby t\u011Bchto DNA-proteinov\u00FDch filament."@cs . "Br\u00E1zdov\u00E1, Marie" . "542394" . "P(GA301/02/0831), P(GA301/99/D078), P(IAA4004110), P(IAA500040513), Z(AV0Z50040507)" . . . "Sequestering of p53 into DNA-protein filaments revealed by electron microscopy" . "2-3" . "Zachycen\u00ED p53 prost\u0159ednictv\u00EDm filament DNA-protein zobrazen\u00E9 elektronovou"@cs . "RIV/68081707:_____/05:00001223!RIV06-AV0-68081707" . . .