"5"^^ . . "Pale\u010Dek, Emil" . "Site-specific chemical modification is a useful technology in characterisation of proteins, but the number of chemical probes of the protein structure reacting with proteins under mild conditions in aqueous solutions is rather limited. Here we studied the reaction of osmium tetroxide, 2,2'-bipyridine (Os,bipy) with several peptides using capillary zone electrophoresis (CZE) and matrix-assisted laser desorption-ionisation-time-of-flight mass spectrometry (MALDI-TOF MS). Both techniques showed formation of a stable complex of Os,bipy with tryptophan residues. In CZE peaks with different migration times and UV-Vis spectra were observed. MALDI-TOF MS showed the formation of a product with characteristic isotopic pattern corresponding to the presence of osmium atom."@en . . "Derivatisation of peptides with osmium tetroxide, 2,2\u00B4-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study"@en . "Site-specific chemical modification is a useful technology in characterisation of proteins, but the number of chemical probes of the protein structure reacting with proteins under mild conditions in aqueous solutions is rather limited. Here we studied the reaction of osmium tetroxide, 2,2'-bipyridine (Os,bipy) with several peptides using capillary zone electrophoresis (CZE) and matrix-assisted laser desorption-ionisation-time-of-flight mass spectrometry (MALDI-TOF MS). Both techniques showed formation of a stable complex of Os,bipy with tryptophan residues. In CZE peaks with different migration times and UV-Vis spectra were observed. MALDI-TOF MS showed the formation of a product with characteristic isotopic pattern corresponding to the presence of osmium atom." . . "Derivatisation of peptides with osmium tetroxide, 2,2\u00B4-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study" . "Billov\u00E1, Sabina" . "Derivatizace peptid\u016F komplexem oxid osmi\u010Del\u00FD, 2,2\u00B4-bipyridin: studie kapil\u00E1rn\u00ED elektrofor\u00E9zou a MALDI-TOF hmotovou spektrometri\u00ED"@cs . . . "Derivatizace peptid\u016F komplexem oxid osmi\u010Del\u00FD, 2,2\u00B4-bipyridin: studie kapil\u00E1rn\u00ED elektrofor\u00E9zou a MALDI-TOF hmotovou spektrometri\u00ED"@cs . "[231859479419]" . "RIV/68081707:_____/04:00104696!RIV/2005/AV0/A17005/N" . "P(GA204/03/0566), P(GA305/03/1100), Z(AV0Z5004920)" . "Havel, J." . "RIV/68081707:_____/04:00104696" . . "9"^^ . . . . . . "261;269" . . . "559787" . "0003-2670" . . "Analytica Chimica Acta" . "\u0160edo, O." . . "2"^^ . "2" . "Derivatisation of peptides with osmium tetroxide, 2,2\u00B4-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study"@en . . . "M\u00EDstn\u011B specifick\u00E1 chemick\u00E1 modifikace je u\u017Eite\u010Dn\u00E1 technologie p\u0159i charakterizaci b\u00EDlkovin. Po\u010Det dostupn\u00FDch chemick\u00FDch sond pro strukturu b\u00EDlkovin, kter\u00E9 reaguj\u00ED s b\u00EDlkovinami za m\u00EDrn\u00FDch podm\u00EDnek ve vodn\u00FDch roztoc\u00EDch, je zna\u010Dn\u011B omezen\u00FD. V t\u00E9to pr\u00E1ci jsme studovali reakci komplexu oxid osmi\u010Del\u00FD, 2,2\u00B4-bipyridin (Os,bipy) s n\u011Bkolika peptidy za vyu\u017Eit\u00ED kapil\u00E1rn\u00ED zon\u00E1ln\u00ED elektrofor\u00E9zy (CZE) a hmotov\u00E9 spektrometrie MALDI-TOF (matrix-assisted laser desorption-ionisation\u2013time-of-flight). Ob\u011Bma technikami byla prok\u00E1z\u00E1na tvorba stabiln\u00EDho komplexu Os,bipy s tryptofanov\u00FDmi zbytky. P\u0159i CZE byly pozorov\u00E1ny p\u00EDky s rozd\u00EDln\u00FDmi dobami putov\u00E1n\u00ED a rozd\u00EDln\u00FDmi spektry UV a viditeln\u00E9 oblasti. MALDI-TOF hmotov\u00E1 spektrometrie prok\u00E1zala tvorbu produktu s charakteristick\u00FDm isotopov\u00FDm obrazem, kter\u00FD odpov\u00EDd\u00E1 p\u0159\u00EDtomnosti osmiov\u00E9ho atomu."@cs . "Derivatisation of peptides with osmium tetroxide, 2,2\u00B4-bipyridine: capillary electrophoretic and MALDI-TOF mass spectrometric study" . "Pe\u0144a-M\u00E9ndez, E. M." . . "515" . "peptide derivatisation; osmium tetroxide; CZE"@en . "NL - Nizozemsko" . .