"Electroanalysis" . . "RIV/68081707:_____/04:00104639" . "P(GA204/03/0566), P(KJB4004302), P(OC D21.002), Z(AV0Z5004920)" . "[CA153A857C8F]" . "Avidin and streptavidin were studied by phase-sensitive AC and cyclic voltammetry as well as by constant current chronopotentiometry at mercury (in alkaline media) and carbon electrodes (in acid medium). In contrast to the generally accepted belief that these proteins are electroinactive, we observed various electrochemical responses at these electrodes. Both proteins produced peaks due to oxidation of tyrosine and tryptophan residues at carbon electrodes and a catalytic peak H at a hanging mercury drop electrode. At the latter electrode avidin produced phase- in AC voltammetric and cyclic voltammetric peaks close to - 0.6 V (peak S) which were assigned to Hg-S interactions, involving cystine/cysteine residues. In cobalt containing solution avidin produced a characteristic catalytic double wave requiring presence of cystine/cysteine residues in the protein molecule."@en . . "Electroactivity of avidin and streptavidin. Avidin signals at mercury and carbon electrodes respond to biotin binding"@en . . . "Havran, Lud\u011Bk" . "Elektroaktivita avidinu a streptavidinu. Vazba biotinu ovliv\u0148uje sign\u00E1ly avidinu na rtu\u0165ov\u00FDch a uhl\u00EDkov\u00FDch elektrod\u00E1ch"@cs . . "Avidin and streptavidin were studied by phase-sensitive AC and cyclic voltammetry as well as by constant current chronopotentiometry at mercury (in alkaline media) and carbon electrodes (in acid medium). In contrast to the generally accepted belief that these proteins are electroinactive, we observed various electrochemical responses at these electrodes. Both proteins produced peaks due to oxidation of tyrosine and tryptophan residues at carbon electrodes and a catalytic peak H at a hanging mercury drop electrode. At the latter electrode avidin produced phase- in AC voltammetric and cyclic voltammetric peaks close to - 0.6 V (peak S) which were assigned to Hg-S interactions, involving cystine/cysteine residues. In cobalt containing solution avidin produced a characteristic catalytic double wave requiring presence of cystine/cysteine residues in the protein molecule." . "Electroactivity of avidin and streptavidin. Avidin signals at mercury and carbon electrodes respond to biotin binding" . . . "1139;1148" . . . . "Electroactivity of avidin and streptavidin. Avidin signals at mercury and carbon electrodes respond to biotin binding" . . . . "streptavidin; avidin-biotin interaction; electrochemical methods"@en . . "Avidin a streptavidin byly studov\u00E1ny pomoc\u00ED f\u00E1zov\u011B citliv\u00E9 AC voltametrie, cyklick\u00E9 voltametrie a chronopotenciometrie s konstantn\u00EDm proudem na rtu\u0165ov\u00FDch (v alkalick\u00E9m prost\u0159ed\u00ED) a uhl\u00EDkov\u00FDch (v kysel\u00E9m prost\u0159ed\u00ED) elektrod\u00E1ch. Na rozd\u00EDl od obecn\u011B p\u0159ij\u00EDman\u00E9ho tvrzen\u00ED, \u017Ee oba proteiny jsou elektroinaktivn\u00ED, byla pozorov\u00E1na \u0159ada r\u016Fzn\u00FDch elektrochemick\u00FDch sign\u00E1l\u016F na obou typech pracovn\u00EDch elektrod. Na uhl\u00EDkov\u00FDch elektrod\u00E1ch poskytuj\u00ED oba proteiny sign\u00E1ly v d\u016Fsledku oxidace aminokyselinov\u00FDch zbytk\u016F tyrozinu a tryptofanu, na vis\u00EDc\u00ED rtu\u0165ov\u00E9 kapkov\u00E9 elektrod\u011B (HMDE) pak sign\u00E1l katalytick\u00E9ho vylu\u010Dov\u00E1n\u00ED vod\u00EDku (p\u00EDk H). Avidin, vzhledem k p\u0159\u00EDtomnosti cysteinu v jeho molekule, poskytuje na HMDE nav\u00EDc tak\u00E9 p\u00EDk p\u0159i potenci\u00E1lu \u20130,6 V (p\u00EDk S) odpov\u00EDdaj\u00EDc\u00ED interakci s\u00EDra \u2013 rtu\u0165. V prost\u0159ed\u00EDch obsahuj\u00EDc\u00EDch kobalt poskytuje avidin charakteristickou katalytickou dvojvlnu vy\u017Eaduj\u00EDc\u00ED p\u0159\u00EDtomnost cysteinov\u00FDch/cystinov\u00FDch aminokyselinov\u00FDch zbytk\u016F v molekule proteinu."@cs . "Billov\u00E1, Sabina" . "1040-0397" . . . "562493" . "Pale\u010Dek, Emil" . "16" . "10"^^ . . . . "13-14" . "3"^^ . "Electroactivity of avidin and streptavidin. Avidin signals at mercury and carbon electrodes respond to biotin binding"@en . "Elektroaktivita avidinu a streptavidinu. Vazba biotinu ovliv\u0148uje sign\u00E1ly avidinu na rtu\u0165ov\u00FDch a uhl\u00EDkov\u00FDch elektrod\u00E1ch"@cs . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "RIV/68081707:_____/04:00104639!RIV/2005/AV0/A17005/N" . . "3"^^ .