"4"^^ . "573461" . "A cathodic stripping transfer voltammetric procedure for trace determination of DNA and its components is described. The method is based on the DNA acid hydrolysis with subsequent electrochemical determination of released purine bases. In the first step, DNA is hydrolyzed for 30 min in 0.5 M perchloric acid at 75 degreesC. The electrochemical step involves generation of Cu(I)-purine base complex on a mercury electrode surface, transfer of electrode with accumulated complex into supporting electrolyte where voltammetric measurement is performed. Analysis is carried out in 14-mul drop volume (two-electrode connection) or in 30-mul drop (three-electrode connection) on a platinum plate, which is used as a counter electrode. Blank electrolyte contains 0.05 M borate buffer, pH 9.2 with 6.3 muM Cu(II). We could observe voltammetric signal at hydrolyzed nucleosides, nucleotides, ODN, and DNA containing purine bases."@en . "4"^^ . . . "Mikroanal\u00FDza DNA pomoc\u00ED rozpou\u0161t\u011Bc\u00ED p\u0159enosov\u00E9 voltametrie"@cs . . . . "Jelen, Franti\u0161ek" . . "4"^^ . . "[B0153C3509E2]" . . . . . . "A cathodic stripping transfer voltammetric procedure for trace determination of DNA and its components is described. The method is based on the DNA acid hydrolysis with subsequent electrochemical determination of released purine bases. In the first step, DNA is hydrolyzed for 30 min in 0.5 M perchloric acid at 75 degreesC. The electrochemical step involves generation of Cu(I)-purine base complex on a mercury electrode surface, transfer of electrode with accumulated complex into supporting electrolyte where voltammetric measurement is performed. Analysis is carried out in 14-mul drop volume (two-electrode connection) or in 30-mul drop (three-electrode connection) on a platinum plate, which is used as a counter electrode. Blank electrolyte contains 0.05 M borate buffer, pH 9.2 with 6.3 muM Cu(II). We could observe voltammetric signal at hydrolyzed nucleosides, nucleotides, ODN, and DNA containing purine bases." . "Microanalysis of DNA by stripping transfer voltammetry"@en . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . "P(GA203/02/0422), P(IAA1163201), P(IBS5004107), Z(AV0Z5004920)" . "Microanalysis of DNA by stripping transfer voltammetry" . "RIV/68081707:_____/04:00104622!RIV/2005/AV0/A17005/N" . . "1567-5394" . . . "Kou\u0159ilov\u00E1, Alena" . . "Microanalysis of DNA by stripping transfer voltammetry" . "Microanalysis of DNA by stripping transfer voltammetry"@en . . "Pe\u010Dinka, Petr" . "DNA determination; determination of purine bases; linear sweep voltammetry"@en . "Pale\u010Dek, Emil" . "RIV/68081707:_____/04:00104622" . . . . "249;252" . "63" . . "Bylo pops\u00E1no pou\u017Eit\u00ED rozpou\u0161t\u011Bc\u00ED katodick\u00E9 p\u0159enosov\u00E9 voltametrick\u00E9 metody pro stanoven\u00ED stopov\u00FDch mno\u017Estv\u00ED DNA a jej\u00EDch komponent. Metoda je zalo\u017Eena na hydrol\u00FDze DNA v kysel\u00E9m prost\u0159ed\u00ED s n\u00E1sledn\u00FDm elektrochemick\u00FDm stanoven\u00EDm uvoln\u011Bn\u00FDch purinov\u00FDch b\u00E1z\u00ED. V prvn\u00EDm kroku byla provedena hydrol\u00FDza v prost\u0159ed\u00ED 0,5 M kyseliny chlorist\u00E9 p\u0159i 75 \u00B0C po dobu 30 min. Elektrochemick\u00FD krok zahrnoval vytvo\u0159en\u00ED komplexu Cu(I)-purinov\u00E1 b\u00E1ze na povrchu rtu\u0165ov\u00E9 elektrody a p\u0159enos elektrody s nahromad\u011Bn\u00FDm komplexem do z\u00E1kladn\u00EDho elektrolytu, kde prob\u011Bhlo voltametrick\u00E9 m\u011B\u0159en\u00ED. Anal\u00FDza byla provedena v kapce vzorku o objemu 14 muL (v dvouelektrodov\u00E9m zapojen\u00ED) nebo 30 muL (v t\u0159\u00EDelektrodov\u00E9m zapojen\u00ED) na platinov\u00E9 podlo\u017Ece, kter\u00E1 slo\u017Eila z\u00E1rove\u0148 jako pomocn\u00E1 elektroda. Z\u00E1kladn\u00ED elektrolyt obsahoval 0,05 M bor\u00E1tov\u00FD pufr s 6,3 muM Cu(II). Voltametrick\u00FD sign\u00E1l byl pozorov\u00E1n u hydrolyzovan\u00FDch nukleotid\u016F, nukleosid\u016F, oligonukleotid\u016F a DNA, pokud vzorky obsahovaly purinov\u00E9 b\u00E1ze."@cs . "Bioelectrochemistry" . "Mikroanal\u00FDza DNA pomoc\u00ED rozpou\u0161t\u011Bc\u00ED p\u0159enosov\u00E9 voltametrie"@cs . "1-2" . .