. . "[E6F9EDD4D9AC]" . . . . "0015-5500" . . "641562" . . "CZ - \u010Cesk\u00E1 republika" . . "Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation."@en . . . "0"^^ . "Folia Biologica" . "P(GA524/99/0694), P(IBS5004009), P(KSK5011112), Z(AV0Z5004920)" . "0"^^ . "Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants."@en . "RIV/68081707:_____/02:17023186" . "Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation." . "4" . "154;156" . . "Kozub\u00EDk, Alois" . . . "5"^^ . . "3"^^ . "48" . "Jan\u00EDk, Robert" . . "Pachern\u00EDk, Ji\u0159\u00ED" . . "Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation." . "Hofmanov\u00E1, Ji\u0159ina" . . "5"^^ . "leukaemia cell line HL-60; electroporation; gene transfection"@en . . . "Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants." . . . "RIV/68081707:_____/02:17023186!RIV/2003/GA0/A17003/N" . "Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation."@en . . . "Bryja, V\u00EDt\u011Bzslav" .