. "Havran, Lud\u011Bk" . . "4"^^ . "0"^^ . "Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393)."@en . "0"^^ . . . "p53; glutathione-S-transferase determination; constant current chronopotentiometry"@en . "RIV/68081707:_____/02:17023049!RIV/2003/GA0/A17003/N" . . "1/2" . "4"^^ . . . "Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393)." . . . "4"^^ . . "Bioelectrochemistry" . . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . . "1567-5394" . . . "Kizek, Ren\u00E9" . "Pale\u010Dek, Emil" . "Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393)." . . . "642721" . "[83292A39FEAB]" . "115;118" . . "Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393)."@en . "P(GA204/00/D049), P(GV204/97/K084), P(IAA4004110), P(NC5343), Z(AV0Z5004920)" . . "Br\u00E1zdov\u00E1, Marie" . . . . "55" . "RIV/68081707:_____/02:17023049" . . "Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample." . . "Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample."@en .