. . . . "RIV/67985939:_____/13:00393243!RIV14-AV0-67985939" . "Cyanobacteria; physiological activity; fluorescent dyes"@en . "Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead."@en . "1932-6203" . "Elster, Josef" . . "12"^^ . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "3"^^ . . . "8" . "10.1371/journal.pone.0055283" . "PLoS ONE" . "1"^^ . "[3171A3D25775]" . . "Tashyreva, D." . . "58918" . "Billi, D." . "I, P(LA341), P(ME 934)" . "RIV/67985939:_____/13:00393243" . . "A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes"@en . . "Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead." . "000315184200014" . . "A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes"@en . "A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes" . . . "A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes" . . "2" .