. . "60" . . "miniature pig; mesenchymal stem cells; cell differentiation"@en . . . "Juh\u00E1sov\u00E1, Jana" . "Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment"@en . . . . "000293138600017" . . "Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment" . . . "Strn\u00E1del, J\u00E1n" . "13"^^ . "CZ - \u010Cesk\u00E1 republika" . "Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation."@en . . "RIV/67985904:_____/11:00367748" . . . . . . "Kl\u00EDma, Ji\u0159\u00ED" . "6"^^ . . "Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment"@en . "P(1M0538), P(2B06130), Z(AV0Z50450515)" . "RIV/67985904:_____/11:00367748!RIV12-AV0-67985904" . "3" . "Physiological Research" . "Motl\u00EDk, Jan" . "Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation." . . . "0862-8408" . "[AECC19365437]" . "Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment" . "Holubov\u00E1, Monika" . "6"^^ . "219038" . . . . . "Juh\u00E1s, \u0160tefan" .