"Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma"@en . . . "Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle \u2013lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility."@en . . "Van Eyk, J." . "978-1-61779-067-6" . "Sheng, S." . . . "397"^^ . "liquid chromatography; chromatofocusing; reversed phase"@en . . "6"^^ . "NEW YORK" . . . . "Z(AV0Z50450515)" . "Skaln\u00EDkov\u00E1, Helena" . "RIV/67985904:_____/11:00359480" . "Fu, Q." . "1"^^ . . "Meng, A." . . "17"^^ . "RIV/67985904:_____/11:00359480!RIV12-AV0-67985904" . "Humana Press" . . "Serum/Plasma Proteomics Metods and Protocols" . . . "205103" . "Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma" . "Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma" . "Everett, A." . "Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle \u2013lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility." . . . "[678B38224CCC]" . "Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma"@en .