"Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development" . "1"^^ . . . "Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development"@en . "Wiley-Blackwell" . "Bhojawni, M." . . . "2"^^ . . "Tomek, W." . . . "Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to e..." . "0936-6768" . "2009-02-26+01:00"^^ . "Smiljakovic, T." . "Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to e..."@en . . . "5"^^ . "Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development" . "Oocyte maturation; Bovine embryo"@en . "Malden" . . "[E10B48D33B5A]" . . "P(GA524/07/1087), Z(AV0Z50450515)" . . "Reproduction in domestic animals" . "RIV/67985904:_____/09:00337404" . . "RIV/67985904:_____/09:00337404!RIV10-AV0-67985904" . "Leipzig" . "346765" . "Kubelka, Michal" . "Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development"@en . . . . "Siemer, C." . .