"000271361500012" . "Tomek, W." . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "[A1F0E0323CAE]" . "Bhojawni, M." . "Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos." . . "1"^^ . . "Kanitz, W." . . . "Molecular Reproduction and Development" . "RIV/67985904:_____/09:00337303" . "Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos."@en . "76" . "Smiljakovic, T." . . . "7"^^ . . . "Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development" . "Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development"@en . "302900" . . "P(GA524/07/1087), Z(AV0Z50450515)" . "12" . "In Vitro maturation; Poly(A) binding protein; Initiation-factor 4E"@en . "12"^^ . . "Kubelka, Michal" . "RIV/67985904:_____/09:00337303!RIV10-AV0-67985904" . "Siemer, C." . . "Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development" . "Analysis of mRNA Associated factors During Bovine Oocyte Maturation and Early Embryonic Development"@en . . . "Leiding, C." . "1040-452X" . . . .