"Chitinolytick\u00FD syst\u00E9m anaerobn\u00EDch polycentrick\u00FDch bachorov\u00FDch hub rodu Orpinomyces a Anaeromyces byl studov\u00E1n ve t\u0159ech frakc\u00EDch- extracelul\u00E1rn\u00ED, cytosolick\u00E9 frakce a frakce z bun\u011B\u010Dn\u00FDch st\u011Bn. Endochitinasa p\u0159edstavovala dominantn\u00ED enzym s nejvy\u0161\u0161\u00ED aktivitou v cytosolick\u00E9 frakci. Endochitinasa u obou rod\u016F byla stabiln\u00ED v rozmez\u00ED pH 4,5 a\u017E 7 s optim\u00E1ln\u00EDm pH 6,5. Endochitinasa u Orpinomyces byla stabiln\u00ED do 50\u00B0C s optim\u00E1ln\u00ED teplotou 50\u00B0C. Endochitinasa z Anaeromyces byla stabiln\u00ED do 40\u00B0C s optim\u00E1ln\u00ED teplotou 40\u00B0C. Nejvhodn\u011Bj\u0161\u00EDm substr\u00E1tem pro endochitinasu u obou rod\u016F byl chitin izolovan\u00FD z houbov\u00FDch bun\u011B\u010Dn\u00FDch st\u011Bn. Enzymov\u00E1 aktivita obou endochitinas byla inhibov\u00E1na iontem Hg2+ a aktivov\u00E1na iontem Mg2+. Ob\u011B endochitinasy byli inhibov\u00E1ny 10 mM SDS a aktivov\u00E1ny iodoacetamidem."@cs . "Folia Microbiologica" . "359578" . "Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide." . "0015-5632" . "RIV/67985904:_____/08:00312643!RIV09-AV0-67985904" . "[1C286AB424B3]" . "\u0160im\u016Fnek, Ji\u0159\u00ED" . "Characterization of chitinases of polycentric anaerobic rumen fungi" . "Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide."@en . "Z(AV0Z50450515)" . . . "3"^^ . . . "3"^^ . . "Characterization of chitinases of polycentric anaerobic rumen fungi" . . "RIV/67985904:_____/08:00312643" . "Charakterizace chitinas polycentrick\u00FDch anaerobn\u00EDch bachorov\u00FDch hub"@cs . . "CZ - \u010Cesk\u00E1 republika" . "3" . "5"^^ . "Fliegerov\u00E1, Kate\u0159ina" . . "Novotn\u00E1, Zuzana" . . "Characterization of chitinases of polycentric anaerobic rumen fungi"@en . . "000257941200014" . "53" . "endochitinase; anaerobic; rumen fungi"@en . . . . . "Charakterizace chitinas polycentrick\u00FDch anaerobn\u00EDch bachorov\u00FDch hub"@cs . "Characterization of chitinases of polycentric anaerobic rumen fungi"@en . . . .