"RIV/67985904:_____/08:00312613!RIV09-AV0-67985904" . "Regulace na \u010Depi\u010Dce z\u00E1visl\u00E9 transla\u010Dn\u00ED iniciace z\u00E1visl\u00E9 v \u010Dasn\u00E9m prase\u010D\u00EDm partenotu"@cs . . "Regulation of cap-dependent translation initiation in the early stage porcine parthenotes"@en . . . . "Regulation of cap-dependent translation initiation in the early stage porcine parthenotes"@en . . "Regulace na \u010Depi\u010Dce z\u00E1visl\u00E9 transla\u010Dn\u00ED iniciace z\u00E1visl\u00E9 v \u010Dasn\u00E9m prase\u010D\u00EDm partenotu"@cs . "P(GA524/04/0104), P(GA524/07/1087), Z(AV0Z50450515)" . "Molecular Reproduction and Development" . "In this study we document that both the translation initiation factor eIF4E and its regulator 4E-BP1 become dephosphorylated in the early stage porcine zygotes already 8 hr post-activation.Similarly, the activities of ERK1/2 MAP and Mnk1 kinases, which are both involved in eIF4E phosphorylation, gradually decrease during this period with the timing similar to that of eIF4E dephosphorylation. The formation of an active eIF4F complex is also diminished after 9-15 hr post-activation, although substantial amounts of this complex have been detected also 24 hr post-activation (2-cell stage). The overall protein synthesis in the parthenotes decreases gradually from 12 hr post-activation reaching a minimum after 48 hr (4-cell stage). Although the translation is gradually decreasing during early preimplantation development, the eIF4F complex, which is temporarily formed, might be a premise for the translation of a small subset of mRNAs at this period of development." . "000260873200004" . . "10"^^ . "7"^^ . . "Regulation of cap-dependent translation initiation in the early stage porcine parthenotes" . . . "12" . "[785C3B76EF9D]" . "In this study we document that both the translation initiation factor eIF4E and its regulator 4E-BP1 become dephosphorylated in the early stage porcine zygotes already 8 hr post-activation.Similarly, the activities of ERK1/2 MAP and Mnk1 kinases, which are both involved in eIF4E phosphorylation, gradually decrease during this period with the timing similar to that of eIF4E dephosphorylation. The formation of an active eIF4F complex is also diminished after 9-15 hr post-activation, although substantial amounts of this complex have been detected also 24 hr post-activation (2-cell stage). The overall protein synthesis in the parthenotes decreases gradually from 12 hr post-activation reaching a minimum after 48 hr (4-cell stage). Although the translation is gradually decreasing during early preimplantation development, the eIF4F complex, which is temporarily formed, might be a premise for the translation of a small subset of mRNAs at this period of development."@en . "Karab\u00EDnov\u00E1, Pavla" . . . "\u0160u\u0161or, Andrej" . . "75" . . "5"^^ . . "Regulation of cap-dependent translation initiation in the early stage porcine parthenotes" . "Kov\u00E1\u0159ov\u00E1, Hana" . "RIV/67985904:_____/08:00312613" . . . "Kubelka, Michal" . . . . "V t\u00E9to studii dokumentujeme, \u017Ee transla\u010Dn\u00ED inicia\u010Dn\u00ED faktor eIF4E a jeho regul\u00E1tor 4E-BP1 jsou defosforylov\u00E1ny v \u010Dasn\u00FDch prase\u010D\u00EDch zygot\u00E1ch ji\u017E za 8 hod po aktivaci. Sou\u010Dasn\u011B se sni\u017Euje aktivita ERK1/2 MAP and Mnk1 kin\u00E1z. Tvorba aktivn\u00EDho eIF4F komplexu je rovn\u011B\u017E sn\u00ED\u017Eena 9-15 hod po aktivaci ale za 24 hod je op\u011Bt detekov\u00E1na jeho podstatn\u00E1 hladina. Proteosynt\u00E9za pr\u016Fb\u011B\u017En\u011B kles\u00E1 do 48 hod.. P\u0159esto\u017Ee je translace v preimplanta\u010Dn\u00EDm stadiu pr\u016Fb\u011B\u017En\u011B sni\u017Eov\u00E1na, m\u016F\u017Ee m\u00EDt p\u0159echodn\u00E1 tvorba eIF4F komplexu vliv na translaci mal\u00E9ho subsetu mRNA v t\u00E9to v\u00FDvojov\u00E9 period\u011B."@cs . "1040-452X" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "meiosis; translation initiation; porcine parthenotes"@en . . "Jel\u00EDnkov\u00E1, Lucie" . "Torner, H." . "Tomek, W." . "392056" .