"RIV/67985904:_____/08:00308694!RIV08-AV0-67985904" . "Utilizing od adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues" . "17"^^ . "Kizek, R." . "Pro stanoven\u00ED metalothionenin\u016F (MT) v melanomov\u00FDch bu\u0148k\u00E1ch, ve zv\u00ED\u0159ec\u00ED melanomov\u00E9 tk\u00E1ni (miniaturn\u00ED prase MeLiM) a v krevn\u00EDm s\u00E9ru pacient\u016F s malign\u00EDm melanomem jsme pou\u017Eili Brdi\u010Dkovu reakci metodou adsorptivn\u00ED p\u0159enosov\u00E9 rozpou\u0161t\u011Bc\u00ED techniky. Obsah MT v bu\u0148k\u00E1ch se pohyboval v rozmez\u00ED od 4.2 do 11.2 \u03BCM. Ve zv\u00ED\u0159ec\u00ED melanomov\u00E9 tk\u00E1ni (melanomy lolalizovan\u00E9 na b\u0159i\u0161e, zadn\u00ED kon\u010Detin\u011B a h\u0159bet\u011B) byl nejvy\u0161\u0161\u00ED obsah MT ur\u010Den ve vzorku z n\u00E1doru na h\u0159bet\u011B a byl t\u00E9m\u011B\u0159 500 \u03BCg MT / g tk\u00E1n\u011B. Tak\u00E9 jsme kvantifikovali MT v metast\u00E1z\u00E1ch, kter\u00E9 byly nalezen\u00E9 v j\u00E1trech, slezin\u011B a lymfatick\u00FDch uzlin\u00E1ch. Pr\u016Fm\u011Brn\u00E1 hladina MT ve vzorc\u00EDch krevn\u00EDch s\u00E9r od pacient\u016F s melanomem byla 3.0 +/- 0.8 \u03BCM. Hladiny MT stanoven\u00E9 v melanomech byly pr\u016Fkazn\u011B (p < 0.05) vy\u0161\u0161\u00ED v porovn\u00E1n\u00ED s kontroln\u00EDmi vzorky bun\u011Bk, tk\u00E1n\u00ED a krevn\u00EDho s\u00E9ra."@cs . . "2"^^ . "3106;3122" . "Strn\u00E1del, J\u00E1n" . "Utilizing od adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues"@en . . "Pou\u017Eit\u00ED Brdi\u010Dkovy reakce metodou adsorptivn\u00ED p\u0159enosov\u00E9 rozpou\u0161t\u011Bc\u00ED techniky pro stanoven\u00ED hladiny metalothionein\u016F v melanomov\u00FDch bu\u0148k\u00E1ch, v krevn\u00EDm s\u00E9ru a ve tk\u00E1n\u00EDch"@cs . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . "We utilized the adsorptive transfer stripping technique Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. The MT content in the cells varied within the range from 4.2 to 11.2 \u03BCM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 \u03BCg of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover, the average MT level in the blood serum samples from patients with melanoma was 3.0 +/- 0.8 \u03BCM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum." . . . "[7B747205A276]" . "Utilizing od adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues"@en . "Chavis, G. J." . . . . . "Adam, V." . . . "10"^^ . "Sensors" . "RIV/67985904:_____/08:00308694" . . "-" . "402008" . . . "Pou\u017Eit\u00ED Brdi\u010Dkovy reakce metodou adsorptivn\u00ED p\u0159enosov\u00E9 rozpou\u0161t\u011Bc\u00ED techniky pro stanoven\u00ED hladiny metalothionein\u016F v melanomov\u00FDch bu\u0148k\u00E1ch, v krevn\u00EDm s\u00E9ru a ve tk\u00E1n\u00EDch"@cs . "Pr\u016F\u0161a, R." . "P(GA524/04/0102), P(IAA600450601), Z(AV0Z50450515)" . . . "Fabrik, I." . "Utilizing od adsorptive transfer stripping technique Brdicka reaction for determination of metallothioneins level in melanoma cells, blood serum and tissues" . . "8" . . "Hor\u00E1k, Vratislav" . . "metallothionein; protein; tumour marker"@en . "Trnkov\u00E1, L." . "Kuka\u010Dka, J." . . "We utilized the adsorptive transfer stripping technique Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. The MT content in the cells varied within the range from 4.2 to 11.2 \u03BCM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 \u03BCg of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover, the average MT level in the blood serum samples from patients with melanoma was 3.0 +/- 0.8 \u03BCM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum."@en . "K\u0159\u00ED\u017Ekov\u00E1, S." . "1424-8220" .